Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 237.54 | 158.78 | p-4EBP1 | 206.20 | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 445.27 | 158.74 | p-Akt | 51.39 | | |
| Triciribine | HCC1954 | 0 (control) | 24 | hr | 327.74 | 105.87 | p-Akt | 62.61 | | |
| Triciribine | HCC1954 | 0 (control) | 6 | hr | 321.42 | 95.62 | p-Akt | 60.23 | | |
| Triciribine | HCC1954 | 1.0 | uM | 6 | hr | 218.47 | 78.02 | p-Akt | 28.41 | |
| Triciribine | MCF 10A | 3.16 | nM | 24 | hr | 267.46 | 146.10 | p-S6 | 248.42 | |
| Triciribine | MCF 10A | 3.16 | nM | 6 | hr | 384.87 | 160.63 | p-S6 | 464.60 | |
| Triciribine | HCC1954 | 1.0 | uM | 24 | hr | 235.33 | 91.33 | p-Akt | 29.69 | |
| Triciribine | AU565 | 0 (control) | 24 | hr | 526.07 | 122.68 | p-Akt | 106.24 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 300.89 | 75.31 | p-Akt | 23.76 | | |
| Triciribine | AU565 | 10.0 | uM | 24 | hr | 427.95 | 65.65 | p-S6 | 116.70 | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 107.57 | 58.92 | p-4EBP1 | 199.41 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 160.29 | 105.55 | p-4EBP1 | 367.55 | |
| Triciribine | HCC1954 | 3.16 | nM | 24 | hr | 320.01 | 105.29 | p-S6 | 296.94 | |
| Triciribine | MCF 10A | 3.16 | uM | 6 | hr | 360.15 | 139.85 | p-4EBP1 | 338.46 | |
| Triciribine | MCF 10A | 3.16 | uM | 24 | hr | 296.35 | 132.49 | p-4EBP1 | 192.33 | |
| Triciribine | HCC1954 | 3.16 | nM | 6 | hr | 275.93 | 96.79 | p-S6 | 400.96 | |
| Triciribine | HCC1954 | 10.0 | uM | 6 | hr | 210.36 | 67.80 | p-Akt | 22.65 | |
| Triciribine | HCC1954 | 10.0 | uM | 24 | hr | 244.11 | 78.00 | p-Akt | 24.77 | |
| Triciribine | AU565 | 10.0 | uM | 6 | hr | 272.99 | 60.30 | p-S6 | 45.49 | |
| Triciribine | T47D | 10.0 | uM | 24 | hr | 195.69 | 74.52 | p-S6 | 435.43 | |
| Triciribine | T47D | 10.0 | uM | 6 | hr | 102.80 | 30.16 | p-S6 | 50.10 | |
| Triciribine | MCF 10A | 10.0 | uM | 24 | hr | 311.22 | 119.59 | p-S6 | 356.95 | |
| Triciribine | AU565 | 100.0 | nM | 6 | hr | 214.70 | 54.97 | p-4EBP1 | 65.17 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 498.81 | 112.95 | p-4EBP1 | 174.19 | |