Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | MCF 10A | 31.6 | nM | 6 | hr | 378.27 | 159.78 | p-Akt | 36.16 | |
| Dactolisib | MCF 10A | 31.6 | nM | 24 | hr | 326.56 | 145.16 | p-Akt | 30.88 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 308.31 | 134.66 | p-Akt | 16.90 | |
| Dactolisib | HCC1954 | 1.0 | uM | 24 | hr | 276.43 | 60.95 | p-S6 | 11.55 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 144.80 | 88.36 | p-Akt | 16.01 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 259.09 | 89.65 | p-4EBP1 | 38.06 | |
| Dactolisib | T47D | 10.0 | uM | 6 | hr | 95.38 | 37.54 | p-Akt | 6.44 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 190.11 | 67.14 | p-4EBP1 | 26.14 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 198.70 | 40.41 | p-S6 | 11.18 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 235.17 | 64.66 | p-Akt | 16.95 | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 218.96 | 75.61 | p-Akt | 41.58 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 88.70 | 45.24 | p-Akt | 10.08 | |
| Dactolisib | T47D | 31.6 | nM | 24 | hr | 113.51 | 47.76 | p-Akt | 26.04 | |
| Dactolisib | HCC1954 | 3.16 | nM | 24 | hr | 294.20 | 99.05 | p-Akt | 76.79 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 494.41 | 109.15 | p-Akt | 109.06 | |
| Dactolisib | T47D | 31.6 | nM | 24 | hr | 112.85 | 54.74 | p-4EBP1 | 222.74 | |
| Dactolisib | MCF 10A | 31.6 | nM | 24 | hr | 276.44 | 153.41 | p-4EBP1 | 89.11 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 90.45 | 41.37 | p-4EBP1 | 104.60 | |
| Dactolisib | HCC1954 | 10.0 | uM | 6 | hr | 273.17 | 84.16 | p-S6 | 19.17 | |
| Dactolisib | HCC1954 | 10.0 | uM | 24 | hr | 271.20 | 74.02 | p-S6 | 8.88 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 142.42 | 100.03 | p-S6 | 6.69 | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 206.58 | 47.55 | p-Akt | 19.83 | | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 527.76 | 112.13 | p-Akt | 112.96 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 211.05 | 49.08 | p-Akt | 19.76 | |