Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 565.14 | 135.83 | p-4EBP1 | 162.84 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 565.94 | 130.77 | p-S6 | 519.83 | | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 566.41 | 105.22 | p-S6 | 313.45 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 567.06 | 138.57 | p-4EBP1 | 168.25 | | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 567.47 | 140.54 | p-4EBP1 | 166.16 | |
| Gefitinib | AU565 | 31.6 | nM | 24 | hr | 567.90 | 126.35 | p-S6 | 538.04 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 571.55 | 130.70 | p-S6 | 558.33 | |
| Gefitinib | AU565 | 10.0 | nM | 24 | hr | 572.61 | 129.37 | p-S6 | 514.02 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 573.43 | 154.46 | p-Akt | 20.79 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 573.89 | 126.37 | p-Akt | 138.88 | | |
| Torkinib | MCF 10A | 10.0 | uM | 6 | hr | 575.82 | 163.71 | p-S6 | 376.86 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 578.56 | 134.27 | p-4EBP1 | 156.00 | |
| Gefitinib | AU565 | 10.0 | nM | 24 | hr | 578.95 | 132.32 | p-4EBP1 | 159.55 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 586.23 | 125.39 | p-Akt | 136.14 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 587.36 | 122.21 | p-Akt | 149.72 | |
| Gefitinib | AU565 | 10.0 | nM | 24 | hr | 588.35 | 123.02 | p-Akt | 145.47 | |
| Torkinib | MCF 10A | 2.37 | uM | 6 | hr | 592.05 | 142.99 | p-S6 | 474.15 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 594.21 | 121.58 | p-S6 | 442.44 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 594.52 | 125.03 | p-Akt | 142.48 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 597.04 | 145.45 | p-4EBP1 | 170.31 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 597.19 | 141.39 | p-4EBP1 | 180.42 | | |
| Torkinib | MCF 10A | 2.37 | uM | 6 | hr | 612.51 | 140.42 | p-4EBP1 | 13.01 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 612.84 | 138.04 | p-S6 | 527.40 | | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 615.73 | 171.90 | p-S6 | 618.02 | |
| Torkinib | MCF 10A | 2.37 | uM | 6 | hr | 620.84 | 155.04 | p-Akt | 19.11 | |