Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | T47D | 10.0 | uM | 6 | hr | 132.61 | 47.26 | p-S6 | 58.99 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 132.66 | 79.82 | p-4EBP1 | 376.65 | |
| Torkinib | T47D | 31.6 | nM | 24 | hr | 132.78 | 57.59 | p-S6 | 16.74 | |
| Torkinib | T47D | 31.6 | nM | 24 | hr | 132.90 | 53.60 | p-S6 | 13.00 | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 133.02 | 96.95 | p-S6 | 277.57 | | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 133.77 | 58.56 | p-Akt | 14.98 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 133.80 | 47.01 | p-4EBP1 | 338.84 | |
| MK2206 | T47D | 1.0 | uM | 24 | hr | 134.17 | 48.49 | p-Akt | 14.42 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 134.56 | 75.54 | p-4EBP1 | 293.71 | |
| Dactolisib | T47D | 10.0 | nM | 24 | hr | 134.63 | 107.17 | p-S6 | 225.51 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 134.86 | 101.30 | p-4EBP1 | 14.17 | |
| MK2206 | T47D | 100.0 | nM | 24 | hr | 135.00 | 67.06 | p-Akt | 17.61 | |
| Gefitinib | HCC1954 | 1.0 | uM | 6 | hr | 135.01 | 33.51 | p-Akt | 20.62 | |
| Gefitinib | HCC1954 | 10.0 | uM | 6 | hr | 135.08 | 44.80 | p-S6 | 62.46 | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 135.21 | 98.09 | p-4EBP1 | 378.64 | |
| Gefitinib | T47D | 316.0 | nM | 24 | hr | 135.34 | 103.12 | p-4EBP1 | 378.08 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 135.75 | 78.36 | p-4EBP1 | 306.59 | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 136.31 | 100.86 | p-4EBP1 | 345.05 | | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 136.33 | 105.43 | p-4EBP1 | 377.18 | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 136.50 | 99.97 | p-S6 | 352.64 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 136.52 | 124.81 | p-4EBP1 | 16.23 | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 136.56 | 81.32 | p-S6 | 300.66 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 136.56 | 74.74 | p-4EBP1 | 384.68 | |
| GSK1059615 | T47D | 100.0 | nM | 24 | hr | 136.59 | 92.08 | p-S6 | 280.44 | |
| MK2206 | T47D | 10.0 | nM | 24 | hr | 136.84 | 105.94 | p-4EBP1 | 360.37 | |