Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | MCF 10A | 7.5 | nM | 24 | hr | 455.61 | 175.00 | p-S6 | 479.38 | |
| GSK1059615 | AU565 | 1.0 | uM | 24 | hr | 455.83 | 72.74 | p-S6 | 22.95 | |
| Triciribine | AU565 | 1.0 | uM | 24 | hr | 456.10 | 90.01 | p-4EBP1 | 126.32 | |
| GSK1059615 | AU565 | 100.0 | nM | 24 | hr | 456.57 | 100.82 | p-S6 | 392.83 | |
| Torkinib | AU565 | 1.78 | nM | 24 | hr | 457.35 | 107.85 | p-S6 | 384.28 | |
| Dactolisib | AU565 | 10.0 | uM | 24 | hr | 457.40 | 71.98 | p-4EBP1 | 10.69 | |
| GSK1059615 | HCC1954 | 10.0 | uM | 6 | hr | 457.84 | 75.13 | p-Akt | 29.14 | |
| Dactolisib | AU565 | 10.0 | nM | 24 | hr | 458.05 | 99.05 | p-4EBP1 | 135.30 | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 458.06 | 113.08 | p-Akt | 91.73 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 458.33 | 123.46 | p-S6 | 585.14 | |
| Triciribine | AU565 | 0 (control) | 24 | hr | 458.45 | 120.14 | p-S6 | 500.89 | | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 458.56 | 155.16 | p-Akt | 49.17 | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 458.85 | 128.23 | p-S6 | 525.22 | | |
| Torkinib | MCF 10A | 1.78 | nM | 6 | hr | 458.96 | 166.90 | p-Akt | 40.68 | |
| Dactolisib | AU565 | 31.6 | nM | 24 | hr | 459.58 | 74.34 | p-Akt | 46.97 | |
| Dactolisib | AU565 | 31.6 | nM | 24 | hr | 459.97 | 76.15 | p-S6 | 19.20 | |
| GSK1059615 | AU565 | 0 (control) | 24 | hr | 460.02 | 114.50 | p-Akt | 98.23 | | |
| Torkinib | MCF 10A | 0.422 | nM | 6 | hr | 460.02 | 163.94 | p-4EBP1 | 355.88 | |
| GSK1059615 | AU565 | 1.0 | uM | 24 | hr | 460.35 | 72.86 | p-Akt | 28.61 | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 461.44 | 121.03 | p-4EBP1 | 176.71 | |
| GSK1059615 | HCC1954 | 3.16 | nM | 6 | hr | 461.88 | 105.26 | p-S6 | 452.98 | |
| GSK1059615 | MCF 10A | 0 (control) | 6 | hr | 462.01 | 162.67 | p-S6 | 489.57 | | |
| Torkinib | AU565 | 0.422 | nM | 24 | hr | 462.08 | 119.67 | p-4EBP1 | 200.90 | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 462.54 | 58.51 | p-S6 | 19.15 | |
| Torkinib | AU565 | 0.422 | nM | 24 | hr | 462.74 | 121.07 | p-4EBP1 | 200.89 | |