Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | AU565 | 0 (control) | 6 | hr | 225.03 | 56.04 | p-4EBP1 | 66.91 | | |
| Triciribine | AU565 | 10.0 | uM | 6 | hr | 252.78 | 56.06 | p-4EBP1 | 47.65 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 280.60 | 56.08 | p-S6 | 32.77 | |
| MK2206 | T47D | 10.0 | uM | 24 | hr | 170.32 | 56.12 | p-4EBP1 | 298.67 | |
| Triciribine | T47D | 3.16 | nM | 6 | hr | 99.80 | 56.12 | p-Akt | 19.64 | |
| Torkinib | AU565 | 0 (control) | 6 | hr | 240.39 | 56.13 | p-4EBP1 | 67.78 | | |
| Triciribine | T47D | 10.0 | nM | 6 | hr | 99.74 | 56.25 | p-4EBP1 | 160.51 | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 234.34 | 56.31 | p-4EBP1 | 61.94 | | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 233.30 | 56.33 | p-Akt | 14.65 | |
| Triciribine | T47D | 10.0 | nM | 6 | hr | 101.35 | 56.34 | p-4EBP1 | 175.96 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 364.25 | 56.36 | p-4EBP1 | 9.92 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 249.08 | 56.47 | p-Akt | 21.82 | |
| MK2206 | HCC1954 | 3.16 | uM | 6 | hr | 181.47 | 56.47 | p-Akt | 13.18 | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 217.10 | 56.50 | p-S6 | 14.26 | |
| Torkinib | HCC1954 | 2.37 | uM | 6 | hr | 268.74 | 56.54 | p-Akt | 13.02 | |
| MK2206 | T47D | 1.0 | uM | 24 | hr | 139.84 | 56.58 | p-4EBP1 | 292.80 | |
| Triciribine | HCC1954 | 3.16 | nM | 6 | hr | 165.07 | 56.66 | p-S6 | 125.32 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 264.17 | 56.83 | p-4EBP1 | 33.37 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 233.58 | 56.94 | p-S6 | 86.02 | |
| MK2206 | HCC1954 | 100.0 | nM | 6 | hr | 182.03 | 57.03 | p-4EBP1 | 78.37 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 228.71 | 57.03 | p-Akt | 21.72 | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 138.70 | 57.11 | p-Akt | 16.15 | |
| GSK1059615 | HCC1954 | 1.0 | uM | 24 | hr | 302.84 | 57.11 | p-Akt | 18.91 | |
| Triciribine | T47D | 10.0 | uM | 24 | hr | 179.90 | 57.17 | p-Akt | 33.20 | |
| Dactolisib | HCC1954 | 3.16 | uM | 6 | hr | 195.19 | 57.19 | p-Akt | 14.48 | |