Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | T47D | 316.0 | nM | 6 | hr | 106.87 | 44.20 | p-4EBP1 | 121.81 | |
| Dactolisib | T47D | 3.16 | uM | 24 | hr | 125.24 | 44.30 | p-S6 | 5.70 | |
| Torkinib | HCC1954 | 0 (control) | 6 | hr | 211.23 | 44.39 | p-4EBP1 | 52.87 | | |
| MK2206 | T47D | 3.16 | uM | 24 | hr | 148.11 | 44.41 | p-S6 | 20.30 | |
| Torkinib | HCC1954 | 0.422 | nM | 6 | hr | 202.89 | 44.42 | p-S6 | 92.64 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 218.19 | 44.53 | p-S6 | 12.18 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 141.77 | 44.53 | p-S6 | 9.54 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 184.48 | 44.56 | p-S6 | 110.17 | |
| Torkinib | AU565 | 7.5 | nM | 6 | hr | 240.99 | 44.76 | p-Akt | 15.24 | |
| Gefitinib | HCC1954 | 10.0 | uM | 6 | hr | 135.08 | 44.80 | p-S6 | 62.46 | |
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 225.60 | 44.84 | p-S6 | 12.00 | |
| GSK1059615 | T47D | 1.0 | uM | 24 | hr | 151.68 | 44.97 | p-Akt | 17.97 | |
| Torkinib | AU565 | 7.5 | nM | 6 | hr | 233.16 | 44.97 | p-S6 | 27.34 | |
| Dactolisib | T47D | 316.0 | nM | 6 | hr | 92.43 | 45.00 | p-Akt | 6.96 | |
| Gefitinib | HCC1954 | 316.0 | nM | 6 | hr | 165.61 | 45.00 | p-4EBP1 | 62.97 | |
| Torkinib | T47D | 31.6 | nM | 24 | hr | 127.17 | 45.02 | p-Akt | 24.28 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 158.33 | 45.19 | p-Akt | 18.61 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 88.70 | 45.24 | p-Akt | 10.08 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 227.70 | 45.24 | p-Akt | 16.61 | |
| Dactolisib | MCF 10A | 1.0 | uM | 24 | hr | 358.73 | 45.31 | p-Akt | 14.31 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 207.63 | 45.48 | p-Akt | 16.31 | |
| Dactolisib | HCC1954 | 316.0 | nM | 6 | hr | 172.52 | 45.49 | p-Akt | 15.11 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 184.63 | 45.52 | p-Akt | 20.62 | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.03 | 45.53 | p-4EBP1 | 121.29 | | |
| Torkinib | HCC1954 | 1.78 | nM | 6 | hr | 177.69 | 45.58 | p-S6 | 95.84 | |