Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 156.03 | 43.22 | p-4EBP1 | 57.72 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 129.60 | 43.22 | p-4EBP1 | 8.96 | |
| Dactolisib | T47D | 316.0 | nM | 24 | hr | 111.62 | 43.30 | p-4EBP1 | 27.62 | |
| Gefitinib | HCC1954 | 10.0 | uM | 6 | hr | 142.98 | 43.33 | p-Akt | 20.79 | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.85 | 43.35 | p-S6 | 72.78 | | |
| MK2206 | T47D | 10.0 | nM | 6 | hr | 95.31 | 43.47 | p-S6 | 46.03 | |
| Dactolisib | HCC1954 | 10.0 | nM | 6 | hr | 162.93 | 43.47 | p-S6 | 11.98 | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 93.80 | 43.48 | p-4EBP1 | 61.08 | |
| MK2206 | T47D | 3.16 | uM | 24 | hr | 148.48 | 43.55 | p-Akt | 13.45 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 200.17 | 43.58 | p-S6 | 11.33 | |
| Torkinib | AU565 | 2.37 | uM | 6 | hr | 242.90 | 43.68 | p-4EBP1 | 7.46 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 194.79 | 43.73 | p-S6 | 15.66 | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 182.86 | 43.74 | p-4EBP1 | 53.60 | | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 114.25 | 43.77 | p-S6 | 32.03 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 123.65 | 43.82 | p-4EBP1 | 9.57 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 145.35 | 43.87 | p-Akt | 26.14 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 146.76 | 43.93 | p-S6 | 6.94 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 144.36 | 44.01 | p-Akt | 26.65 | |
| Dactolisib | T47D | 3.16 | uM | 6 | hr | 94.75 | 44.06 | p-Akt | 6.83 | |
| MK2206 | T47D | 3.16 | uM | 24 | hr | 150.54 | 44.09 | p-Akt | 14.26 | |
| Torkinib | T47D | 562.0 | nM | 24 | hr | 117.11 | 44.11 | p-4EBP1 | 29.15 | |
| MK2206 | T47D | 316.0 | nM | 6 | hr | 97.21 | 44.14 | p-4EBP1 | 106.54 | |
| Torkinib | T47D | 2.37 | uM | 24 | hr | 125.67 | 44.17 | p-4EBP1 | 13.31 | |
| Triciribine | T47D | 3.16 | uM | 6 | hr | 104.79 | 44.18 | p-S6 | 46.47 | |
| Torkinib | AU565 | 562.0 | nM | 6 | hr | 250.73 | 44.18 | p-4EBP1 | 7.70 | |