Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | MCF 10A | 3.16 | uM | 6 | hr | 369.53 | 146.17 | p-Akt | 17.62 | |
| Dactolisib | HCC1954 | 100.0 | nM | 24 | hr | 277.05 | 85.11 | p-S6 | 17.62 | |
| GSK1059615 | AU565 | 1.0 | uM | 6 | hr | 216.34 | 42.62 | p-Akt | 17.65 | |
| Triciribine | MCF 10A | 3.16 | uM | 6 | hr | 370.92 | 146.93 | p-Akt | 17.67 | |
| Gefitinib | MCF 10A | 1.0 | uM | 24 | hr | 158.54 | 29.78 | p-Akt | 17.71 | |
| GSK1059615 | AU565 | 3.16 | uM | 6 | hr | 328.03 | 69.27 | p-Akt | 17.72 | |
| GSK1059615 | MCF 10A | 3.16 | uM | 24 | hr | 206.97 | 114.82 | p-4EBP1 | 17.75 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 327.69 | 72.03 | p-Akt | 17.75 | |
| GSK1059615 | HCC1954 | 3.16 | uM | 24 | hr | 297.52 | 63.23 | p-4EBP1 | 17.76 | |
| Triciribine | MCF 10A | 3.16 | uM | 24 | hr | 291.55 | 118.36 | p-Akt | 17.80 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 395.02 | 66.41 | p-Akt | 17.83 | |
| Torkinib | HCC1954 | 562.0 | nM | 24 | hr | 239.91 | 70.30 | p-Akt | 17.84 | |
| Triciribine | MCF 10A | 100.0 | nM | 24 | hr | 251.10 | 142.37 | p-Akt | 17.85 | |
| Triciribine | AU565 | 31.6 | nM | 6 | hr | 220.69 | 55.70 | p-Akt | 17.86 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 317.65 | 70.54 | p-Akt | 17.86 | |
| Dactolisib | HCC1954 | 3.16 | uM | 6 | hr | 270.38 | 74.22 | p-Akt | 17.89 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 255.42 | 63.44 | p-Akt | 17.92 | |
| Torkinib | MCF 10A | 2.37 | uM | 6 | hr | 561.92 | 142.49 | p-Akt | 17.93 | |
| GSK1059615 | T47D | 1.0 | uM | 24 | hr | 151.68 | 44.97 | p-Akt | 17.97 | |
| Dactolisib | HCC1954 | 10.0 | uM | 24 | hr | 288.74 | 69.44 | p-Akt | 18.00 | |
| MK2206 | MCF 10A | 1.0 | uM | 6 | hr | 378.15 | 147.34 | p-Akt | 18.05 | |
| MK2206 | HCC1954 | 3.16 | uM | 6 | hr | 220.00 | 47.07 | p-Akt | 18.05 | |
| Triciribine | AU565 | 100.0 | nM | 6 | hr | 255.98 | 65.84 | p-Akt | 18.08 | |
| Gefitinib | HCC1954 | 0 (control) | 6 | hr | 184.31 | 43.16 | p-Akt | 18.10 | | |
| GSK1059615 | AU565 | 10.0 | uM | 24 | hr | 323.29 | 73.24 | p-Akt | 18.11 | |