Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | T47D | 1.0 | uM | 24 | hr | 160.74 | 72.11 | p-S6 | 440.48 | |
| Gefitinib | T47D | 3.16 | nM | 6 | hr | 191.33 | 107.09 | p-S6 | 441.08 | |
| Gefitinib | AU565 | 316.0 | nM | 24 | hr | 422.43 | 94.36 | p-S6 | 441.87 | |
| Gefitinib | T47D | 10.0 | uM | 6 | hr | 154.80 | 87.88 | p-4EBP1 | 442.33 | |
| MK2206 | AU565 | 31.6 | nM | 24 | hr | 405.92 | 121.43 | p-S6 | 442.43 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 594.21 | 121.58 | p-S6 | 442.44 | |
| GSK1059615 | HCC1954 | 10.0 | nM | 6 | hr | 497.92 | 100.21 | p-S6 | 442.56 | |
| Gefitinib | T47D | 1.0 | nM | 6 | hr | 199.89 | 107.14 | p-S6 | 443.29 | |
| Torkinib | T47D | 0 (control) | 6 | hr | 189.83 | 98.37 | p-S6 | 444.84 | | |
| Triciribine | T47D | 316.0 | nM | 24 | hr | 172.69 | 87.09 | p-S6 | 445.42 | |
| Triciribine | HCC1954 | 10.0 | uM | 24 | hr | 211.66 | 90.86 | p-S6 | 445.45 | |
| Gefitinib | T47D | 0 (control) | 6 | hr | 193.18 | 93.79 | p-4EBP1 | 445.45 | | |
| MK2206 | MCF 10A | 31.6 | nM | 6 | hr | 385.30 | 156.59 | p-S6 | 445.48 | |
| Gefitinib | T47D | 1.0 | nM | 6 | hr | 186.22 | 98.66 | p-S6 | 445.75 | |
| GSK1059615 | AU565 | 100.0 | nM | 24 | hr | 479.81 | 110.27 | p-S6 | 446.19 | |
| GSK1059615 | T47D | 10.0 | nM | 6 | hr | 182.36 | 97.77 | p-S6 | 446.34 | |
| Gefitinib | AU565 | 316.0 | nM | 24 | hr | 435.55 | 101.60 | p-S6 | 446.57 | |
| Gefitinib | MCF 10A | 100.0 | nM | 6 | hr | 352.15 | 168.35 | p-S6 | 446.69 | |
| Gefitinib | MCF 10A | 31.6 | nM | 6 | hr | 393.79 | 169.73 | p-S6 | 446.82 | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 385.84 | 157.07 | p-S6 | 446.88 | |
| Torkinib | HCC1954 | 31.6 | nM | 24 | hr | 248.45 | 102.30 | p-S6 | 448.86 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 24 | hr | 455.51 | 172.86 | p-S6 | 451.67 | |
| Gefitinib | MCF 10A | 1.0 | nM | 6 | hr | 424.19 | 165.67 | p-S6 | 452.39 | |
| GSK1059615 | HCC1954 | 3.16 | nM | 6 | hr | 461.88 | 105.26 | p-S6 | 452.98 | |
| GSK1059615 | T47D | 100.0 | nM | 6 | hr | 164.04 | 98.99 | p-S6 | 454.45 | |