Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 209.97 | 51.77 | p-4EBP1 | 55.32 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 313.57 | 67.91 | p-Akt | 32.33 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 264.89 | 62.49 | p-4EBP1 | 79.32 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 215.94 | 45.64 | p-S6 | 67.64 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 253.13 | 58.08 | p-S6 | 23.07 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 226.23 | 50.86 | p-4EBP1 | 51.79 | | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 221.28 | 52.62 | p-Akt | 23.51 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 270.31 | 60.18 | p-4EBP1 | 67.03 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 266.21 | 59.03 | p-4EBP1 | 67.26 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 308.09 | 68.07 | p-Akt | 33.62 | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 235.49 | 54.01 | p-Akt | 23.90 | | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 218.03 | 53.42 | p-4EBP1 | 64.33 | | |
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 334.11 | 66.21 | p-4EBP1 | 14.45 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 261.82 | 58.53 | p-Akt | 20.86 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 200.17 | 43.58 | p-S6 | 11.33 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 296.50 | 62.96 | p-4EBP1 | 8.36 | |
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 269.91 | 55.81 | p-S6 | 12.02 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 377.06 | 85.43 | p-4EBP1 | 9.63 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 260.00 | 60.48 | p-4EBP1 | 77.46 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 259.31 | 60.60 | p-Akt | 24.45 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 217.68 | 50.97 | p-S6 | 71.68 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 317.65 | 70.54 | p-Akt | 17.86 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 244.28 | 53.82 | p-S6 | 11.73 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 250.79 | 51.61 | p-Akt | 14.99 | |
| Dactolisib | AU565 | 10.0 | uM | 6 | hr | 257.48 | 55.92 | p-4EBP1 | 8.48 | |