Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 246.69 | 58.06 | p-S6 | 11.73 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 218.57 | 50.64 | p-4EBP1 | 58.44 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 249.95 | 67.43 | p-S6 | 79.51 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 292.18 | 65.87 | p-Akt | 21.37 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 329.62 | 68.74 | p-Akt | 34.37 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 269.00 | 60.51 | p-S6 | 148.29 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 305.86 | 66.00 | p-S6 | 165.72 | | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 234.22 | 57.81 | p-Akt | 20.79 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 281.51 | 66.62 | p-Akt | 23.00 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 297.48 | 65.22 | p-Akt | 31.78 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 262.04 | 61.46 | p-Akt | 19.52 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 191.61 | 37.14 | p-S6 | 9.87 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 191.08 | 38.65 | p-Akt | 14.78 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 236.79 | 58.62 | p-4EBP1 | 75.30 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 232.04 | 50.38 | p-4EBP1 | 56.29 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 229.96 | 58.85 | p-S6 | 64.24 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 239.03 | 48.95 | p-4EBP1 | 49.90 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 331.93 | 71.96 | p-S6 | 204.40 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 267.09 | 64.29 | p-S6 | 140.82 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 230.66 | 50.40 | p-S6 | 113.96 | | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 214.01 | 52.78 | p-4EBP1 | 62.72 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 216.77 | 47.93 | p-4EBP1 | 49.07 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 303.82 | 65.62 | p-4EBP1 | 88.27 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 269.00 | 63.11 | p-S6 | 144.00 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 286.12 | 61.91 | p-Akt | 30.06 | |