Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 205.49 | 54.71 | p-Akt | 18.44 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 416.27 | 84.19 | p-S6 | 167.32 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 280.60 | 56.08 | p-S6 | 32.77 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 319.11 | 82.93 | p-S6 | 274.09 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 311.09 | 61.50 | p-4EBP1 | 70.16 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 268.49 | 73.16 | p-4EBP1 | 98.25 | | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 304.34 | 68.30 | p-S6 | 77.10 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 187.44 | 52.00 | p-Akt | 19.13 | | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 323.13 | 77.03 | p-4EBP1 | 111.42 | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 358.63 | 76.79 | p-4EBP1 | 131.88 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 225.66 | 66.86 | p-Akt | 22.58 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 232.08 | 52.54 | p-S6 | 24.57 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 257.70 | 53.65 | p-Akt | 14.45 | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 343.27 | 71.17 | p-4EBP1 | 102.55 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 368.31 | 89.82 | p-S6 | 319.52 | |
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 400.16 | 81.54 | p-Akt | 34.73 | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 255.74 | 64.02 | p-4EBP1 | 86.03 | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 335.20 | 72.50 | p-S6 | 103.10 | |
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 290.33 | 70.47 | p-4EBP1 | 87.48 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 347.95 | 72.52 | p-4EBP1 | 121.39 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 260.81 | 58.97 | p-Akt | 13.43 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 333.41 | 71.95 | p-4EBP1 | 113.09 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 368.63 | 75.60 | p-Akt | 17.45 | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 207.92 | 51.79 | p-Akt | 14.32 | |