Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 352.21 | 85.33 | p-S6 | 323.62 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 371.15 | 65.46 | p-4EBP1 | 92.53 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 217.60 | 53.69 | p-Akt | 13.47 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 202.91 | 61.14 | p-S6 | 108.44 | | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 302.08 | 70.82 | p-4EBP1 | 107.19 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 371.49 | 74.30 | p-4EBP1 | 132.74 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 360.15 | 72.47 | p-4EBP1 | 128.99 | |
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 340.94 | 77.61 | p-4EBP1 | 117.81 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 363.52 | 69.11 | p-Akt | 16.75 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 316.22 | 67.25 | p-4EBP1 | 94.25 | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 395.42 | 73.69 | p-4EBP1 | 128.53 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 332.05 | 77.65 | p-S6 | 125.85 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 265.59 | 64.05 | p-S6 | 81.69 | |
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 318.99 | 83.12 | p-S6 | 233.34 | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 395.87 | 81.88 | p-Akt | 27.47 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 273.26 | 74.15 | p-4EBP1 | 88.08 | | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 311.60 | 70.51 | p-S6 | 88.93 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 255.60 | 70.38 | p-Akt | 22.59 | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 321.05 | 67.77 | p-Akt | 15.69 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 355.42 | 84.04 | p-4EBP1 | 122.33 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 364.01 | 73.26 | p-Akt | 18.17 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 254.16 | 70.09 | p-S6 | 156.06 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 291.42 | 63.83 | p-S6 | 65.88 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 277.65 | 78.69 | p-S6 | 159.10 | | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 342.27 | 81.34 | p-S6 | 213.56 | |