Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | MCF 10A | 1.0 | uM | 6 | hr | 371.64 | 151.24 | p-Akt | 19.17 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 24 | hr | 270.51 | 77.62 | p-Akt | 15.17 | |
| Triciribine | T47D | 1.0 | uM | 24 | hr | 178.41 | 84.59 | p-S6 | 460.89 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 6 | hr | 215.88 | 128.98 | p-Akt | 16.35 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 424.14 | 125.45 | p-4EBP1 | 174.34 | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 89.72 | 38.09 | p-S6 | 3.89 | |
| MK2206 | T47D | 1.0 | nM | 6 | hr | 94.42 | 35.53 | p-Akt | 13.06 | |
| Gefitinib | MCF 10A | 1.0 | uM | 24 | hr | 158.54 | 29.78 | p-Akt | 17.71 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| Gefitinib | MCF 10A | 1.0 | uM | 6 | hr | 183.24 | 141.46 | p-Akt | 28.84 | |
| Dactolisib | HCC1954 | 1.0 | uM | 24 | hr | 288.44 | 57.74 | p-Akt | 18.48 | |
| Triciribine | T47D | 1.0 | uM | 6 | hr | 121.20 | 71.28 | p-S6 | 120.93 | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 108.89 | 34.29 | p-S6 | 4.77 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 235.17 | 64.66 | p-Akt | 16.95 | |
| Triciribine | HCC1954 | 1.0 | uM | 24 | hr | 235.33 | 91.33 | p-Akt | 29.69 | |
| GSK1059615 | T47D | 1.0 | nM | 6 | hr | 177.09 | 87.31 | p-Akt | 93.26 | |
| Triciribine | HCC1954 | 1.0 | uM | 6 | hr | 218.47 | 78.02 | p-Akt | 28.41 | |
| MK2206 | HCC1954 | 1.0 | uM | 24 | hr | 242.00 | 86.91 | p-Akt | 18.32 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 114.25 | 43.77 | p-S6 | 32.03 | |
| MK2206 | HCC1954 | 1.0 | uM | 6 | hr | 154.63 | 35.58 | p-Akt | 11.74 | |
| GSK1059615 | HCC1954 | 1.0 | uM | 24 | hr | 289.77 | 58.56 | p-Akt | 18.53 | |
| GSK1059615 | HCC1954 | 1.0 | uM | 6 | hr | 340.19 | 79.16 | p-Akt | 19.95 | |
| GSK1059615 | T47D | 1.0 | nM | 24 | hr | 163.58 | 90.72 | p-Akt | 66.78 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 492.56 | 131.78 | p-4EBP1 | 221.65 | |
| MK2206 | T47D | 1.0 | uM | 24 | hr | 152.27 | 69.95 | p-S6 | 79.39 | |