Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 403.53 | 59.74 | p-Akt | 21.21 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 24 | hr | 306.73 | 152.32 | p-S6 | 257.52 | |
| MK2206 | MCF 10A | 316.0 | nM | 6 | hr | 402.65 | 157.16 | p-S6 | 488.56 | |
| MK2206 | MCF 10A | 316.0 | nM | 24 | hr | 287.84 | 160.35 | p-S6 | 267.15 | |
| Triciribine | MCF 10A | 316.0 | nM | 6 | hr | 405.80 | 158.51 | p-S6 | 492.45 | |
| Triciribine | MCF 10A | 316.0 | nM | 24 | hr | 235.13 | 150.80 | p-S6 | 216.81 | |
| Dactolisib | MCF 10A | 316.0 | nM | 6 | hr | 360.93 | 123.35 | p-S6 | 338.83 | |
| Gefitinib | HCC1954 | 316.0 | nM | 6 | hr | 169.38 | 48.39 | p-Akt | 24.53 | |
| Gefitinib | HCC1954 | 316.0 | nM | 24 | hr | 250.36 | 103.57 | p-Akt | 72.98 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 332.74 | 49.60 | p-S6 | 216.41 | |
| Gefitinib | T47D | 316.0 | nM | 6 | hr | 169.95 | 110.41 | p-S6 | 492.09 | |
| Gefitinib | T47D | 316.0 | nM | 24 | hr | 146.68 | 117.39 | p-S6 | 365.01 | |
| GSK1059615 | T47D | 316.0 | nM | 6 | hr | 165.06 | 78.53 | p-S6 | 39.67 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 141.77 | 44.53 | p-S6 | 9.54 | |
| MK2206 | T47D | 316.0 | nM | 6 | hr | 110.20 | 48.08 | p-S6 | 40.07 | |
| Dactolisib | T47D | 316.0 | nM | 24 | hr | 111.62 | 43.30 | p-4EBP1 | 27.62 | |
| Dactolisib | T47D | 316.0 | nM | 6 | hr | 97.57 | 46.09 | p-4EBP1 | 19.39 | |
| Triciribine | T47D | 316.0 | nM | 24 | hr | 157.83 | 82.22 | p-4EBP1 | 351.16 | |
| Triciribine | T47D | 316.0 | nM | 6 | hr | 111.77 | 48.64 | p-4EBP1 | 161.35 | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 130.59 | 62.14 | p-4EBP1 | 310.05 | |
| MK2206 | T47D | 316.0 | nM | 6 | hr | 106.87 | 44.20 | p-4EBP1 | 121.81 | |
| GSK1059615 | T47D | 316.0 | nM | 24 | hr | 133.80 | 47.01 | p-4EBP1 | 338.84 | |
| GSK1059615 | T47D | 316.0 | nM | 6 | hr | 150.58 | 73.05 | p-4EBP1 | 497.11 | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 144.25 | 90.81 | p-S6 | 291.15 | |
| Triciribine | T47D | 316.0 | nM | 6 | hr | 119.23 | 59.88 | p-S6 | 87.34 | |