Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | T47D | 0.1 | nM | 6 | hr | 173.65 | 92.27 | p-Akt | 90.58 | |
| GSK1059615 | T47D | 1.0 | nM | 24 | hr | 163.58 | 90.72 | p-Akt | 66.78 | |
| MK2206 | T47D | 1.0 | nM | 6 | hr | 94.42 | 35.53 | p-Akt | 13.06 | |
| Triciribine | T47D | 1.0 | nM | 6 | hr | 96.98 | 46.31 | p-Akt | 19.11 | |
| Triciribine | T47D | 1.0 | nM | 24 | hr | 146.78 | 89.33 | p-Akt | 59.46 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 230.65 | 52.24 | p-Akt | 23.88 | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 484.31 | 116.72 | p-Akt | 84.98 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 428.83 | 103.88 | p-Akt | 86.48 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 225.66 | 66.86 | p-Akt | 22.58 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 484.33 | 118.39 | p-Akt | 103.66 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 322.82 | 72.03 | p-Akt | 33.80 | |
| Gefitinib | HCC1954 | 1.0 | nM | 24 | hr | 309.20 | 115.84 | p-Akt | 81.59 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 24 | hr | 316.53 | 106.00 | p-Akt | 70.73 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 158.33 | 45.19 | p-Akt | 18.61 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 192.23 | 55.17 | p-Akt | 31.66 | |
| Triciribine | HCC1954 | 1.0 | nM | 24 | hr | 295.65 | 96.48 | p-Akt | 59.73 | |
| Gefitinib | MCF 10A | 1.0 | nM | 6 | hr | 434.84 | 165.60 | p-Akt | 49.10 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 349.56 | 127.22 | p-Akt | 57.16 | |
| Torkinib | MCF 10A | 0.1 | nM | 24 | hr | 342.29 | 124.00 | p-Akt | 50.29 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 6 | hr | 470.40 | 164.01 | p-Akt | 53.70 | |
| Triciribine | MCF 10A | 1.0 | nM | 24 | hr | 311.02 | 118.46 | p-Akt | 43.92 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 297.48 | 65.22 | p-Akt | 31.78 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 556.22 | 122.40 | p-Akt | 136.15 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 586.23 | 125.39 | p-Akt | 136.14 | |