Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | T47D | 0 (control) | 6 | hr | 203.12 | 105.90 | p-S6 | 455.20 | | |
| Dactolisib | T47D | 31.6 | nM | 24 | hr | 113.51 | 47.76 | p-Akt | 26.04 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 88.70 | 45.24 | p-Akt | 10.08 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 173.98 | 88.45 | p-Akt | 50.47 | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 111.54 | 58.38 | p-Akt | 17.15 | |
| MK2206 | T47D | 31.6 | nM | 24 | hr | 146.52 | 86.34 | p-Akt | 26.31 | |
| MK2206 | T47D | 31.6 | nM | 6 | hr | 105.79 | 49.19 | p-Akt | 9.27 | |
| GSK1059615 | T47D | 31.6 | nM | 24 | hr | 155.80 | 86.40 | p-Akt | 49.01 | |
| GSK1059615 | T47D | 31.6 | nM | 6 | hr | 168.17 | 85.32 | p-Akt | 73.10 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 144.08 | 65.96 | p-Akt | 33.04 | |
| Torkinib | T47D | 7.5 | nM | 6 | hr | 157.46 | 86.43 | p-Akt | 48.47 | |
| Gefitinib | T47D | 31.6 | nM | 24 | hr | 155.72 | 101.88 | p-Akt | 76.48 | |
| Gefitinib | T47D | 31.6 | nM | 6 | hr | 167.03 | 97.05 | p-Akt | 101.76 | |
| Gefitinib | T47D | 10.0 | uM | 6 | hr | 154.80 | 87.88 | p-4EBP1 | 442.33 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 136.56 | 74.74 | p-4EBP1 | 384.68 | |
| Torkinib | T47D | 10.0 | uM | 6 | hr | 151.47 | 67.96 | p-4EBP1 | 20.79 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 129.60 | 43.22 | p-4EBP1 | 8.96 | |
| GSK1059615 | T47D | 10.0 | uM | 6 | hr | 182.18 | 64.63 | p-4EBP1 | 61.63 | |
| GSK1059615 | T47D | 10.0 | uM | 24 | hr | 192.30 | 72.79 | p-4EBP1 | 85.63 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 105.81 | 29.63 | p-4EBP1 | 65.78 | |
| MK2206 | T47D | 10.0 | uM | 24 | hr | 171.15 | 53.35 | p-4EBP1 | 280.74 | |
| Triciribine | T47D | 10.0 | uM | 6 | hr | 103.50 | 31.15 | p-4EBP1 | 87.59 | |
| Triciribine | T47D | 10.0 | uM | 24 | hr | 155.73 | 64.52 | p-4EBP1 | 343.55 | |
| Dactolisib | T47D | 10.0 | uM | 6 | hr | 85.23 | 24.35 | p-4EBP1 | 5.42 | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 134.86 | 101.30 | p-4EBP1 | 14.17 | |