Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 290.65 | 59.15 | p-Akt | 17.08 | |
| Torkinib | AU565 | 0.422 | nM | 6 | hr | 372.97 | 73.36 | p-4EBP1 | 96.01 | |
| Dactolisib | AU565 | 100.0 | nM | 24 | hr | 377.63 | 60.16 | p-Akt | 25.52 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 515.85 | 125.87 | p-S6 | 463.78 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 235.86 | 67.93 | p-S6 | 136.84 | |
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 399.01 | 123.18 | p-S6 | 489.38 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 525.60 | 117.07 | p-S6 | 425.59 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 206.88 | 49.24 | p-S6 | 116.08 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 266.08 | 62.37 | p-4EBP1 | 79.48 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 471.12 | 120.05 | p-4EBP1 | 173.15 | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 427.04 | 124.23 | p-Akt | 78.88 | | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 291.94 | 77.13 | p-4EBP1 | 98.69 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 597.04 | 145.45 | p-4EBP1 | 170.31 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 289.01 | 63.74 | p-4EBP1 | 68.42 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 268.55 | 59.48 | p-S6 | 131.60 | |
| Torkinib | AU565 | 0.422 | nM | 6 | hr | 297.99 | 64.59 | p-S6 | 137.32 | |
| Torkinib | AU565 | 0.422 | nM | 24 | hr | 486.10 | 119.56 | p-S6 | 478.18 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 261.27 | 65.61 | p-4EBP1 | 75.05 | |
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 416.57 | 131.35 | p-4EBP1 | 192.89 | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 484.96 | 127.02 | p-4EBP1 | 215.15 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 346.86 | 73.14 | p-4EBP1 | 98.91 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| Triciribine | AU565 | 10.0 | nM | 6 | hr | 205.52 | 54.30 | p-4EBP1 | 68.04 | |
| MK2206 | AU565 | 10.0 | nM | 24 | hr | 384.71 | 118.72 | p-4EBP1 | 187.22 | |