Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | AU565 | 1.78 | nM | 24 | hr | 442.08 | 102.06 | p-Akt | 74.88 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 215.16 | 63.34 | p-Akt | 22.81 | | |
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 183.97 | 46.00 | p-Akt | 14.47 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 286.03 | 62.73 | p-Akt | 29.07 | | |
| Triciribine | AU565 | 10.0 | nM | 6 | hr | 278.40 | 74.05 | p-Akt | 23.07 | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 427.04 | 124.23 | p-Akt | 78.88 | | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 659.08 | 130.41 | p-Akt | 134.35 | | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 522.19 | 120.45 | p-Akt | 94.97 | | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 230.65 | 52.24 | p-Akt | 23.88 | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 231.71 | 61.01 | p-Akt | 21.54 | | |
| Torkinib | AU565 | 0.422 | nM | 24 | hr | 481.77 | 110.68 | p-Akt | 88.17 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 556.22 | 122.40 | p-Akt | 136.15 | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 458.06 | 113.08 | p-Akt | 91.73 | |
| Triciribine | AU565 | 0 (control) | 24 | hr | 511.74 | 118.54 | p-Akt | 102.35 | | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 428.83 | 103.88 | p-Akt | 86.48 | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 264.31 | 61.82 | p-Akt | 28.75 | | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 484.31 | 116.72 | p-Akt | 84.98 | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 235.49 | 54.01 | p-Akt | 23.90 | | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 225.66 | 66.86 | p-Akt | 22.58 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 225.05 | 59.35 | p-Akt | 21.63 | |
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 380.47 | 122.42 | p-Akt | 68.40 | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 496.14 | 121.03 | p-Akt | 137.91 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 297.48 | 65.22 | p-Akt | 31.78 | |
| Gefitinib | AU565 | 31.6 | nM | 24 | hr | 497.62 | 115.28 | p-Akt | 129.27 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 224.67 | 51.67 | p-Akt | 24.54 | |