Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 277.70 | 67.19 | p-4EBP1 | 80.51 | |
| Torkinib | AU565 | 31.6 | nM | 6 | hr | 251.01 | 49.47 | p-4EBP1 | 31.81 | |
| Torkinib | AU565 | 31.6 | nM | 24 | hr | 395.95 | 75.66 | p-4EBP1 | 84.49 | |
| Dactolisib | AU565 | 10.0 | uM | 24 | hr | 452.82 | 70.98 | p-Akt | 21.40 | |
| MK2206 | AU565 | 100.0 | nM | 24 | hr | 354.53 | 104.31 | p-4EBP1 | 151.28 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 498.81 | 112.95 | p-4EBP1 | 174.19 | |
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 330.98 | 64.06 | p-4EBP1 | 14.64 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 257.70 | 53.65 | p-Akt | 14.45 | |
| GSK1059615 | AU565 | 10.0 | uM | 24 | hr | 327.44 | 83.88 | p-Akt | 18.75 | |
| MK2206 | AU565 | 10.0 | uM | 24 | hr | 331.32 | 68.08 | p-Akt | 18.16 | |
| Triciribine | AU565 | 10.0 | uM | 6 | hr | 331.62 | 71.61 | p-Akt | 21.64 | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 231.71 | 61.01 | p-Akt | 21.54 | | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 234.01 | 54.85 | p-Akt | 22.11 | |
| Gefitinib | AU565 | 316.0 | nM | 24 | hr | 399.13 | 99.71 | p-Akt | 107.30 | |
| Torkinib | AU565 | 133.0 | nM | 6 | hr | 314.14 | 57.99 | p-Akt | 14.98 | |
| Torkinib | AU565 | 133.0 | nM | 24 | hr | 395.71 | 61.17 | p-Akt | 22.66 | |
| GSK1059615 | AU565 | 316.0 | nM | 6 | hr | 232.75 | 48.96 | p-Akt | 23.52 | |
| GSK1059615 | AU565 | 316.0 | nM | 24 | hr | 474.59 | 87.91 | p-Akt | 63.03 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 266.67 | 61.68 | p-Akt | 13.97 | |
| MK2206 | AU565 | 316.0 | nM | 24 | hr | 320.76 | 76.81 | p-Akt | 22.30 | |
| Triciribine | AU565 | 316.0 | nM | 6 | hr | 344.20 | 83.25 | p-Akt | 21.62 | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 468.57 | 85.00 | p-Akt | 42.85 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 300.22 | 59.06 | p-Akt | 16.85 | |
| Dactolisib | AU565 | 31.6 | nM | 24 | hr | 459.97 | 76.15 | p-S6 | 19.20 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 214.57 | 42.34 | p-S6 | 13.61 | |