Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 100.0 | nM | 24 | hr | 377.63 | 60.16 | p-Akt | 25.52 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 424.14 | 125.45 | p-4EBP1 | 174.34 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 261.27 | 65.61 | p-4EBP1 | 75.05 | |
| Torkinib | AU565 | 0.422 | nM | 6 | hr | 297.99 | 64.59 | p-S6 | 137.32 | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 427.04 | 124.23 | p-Akt | 78.88 | | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 403.53 | 59.74 | p-Akt | 21.21 | |
| Torkinib | AU565 | 10.0 | uM | 24 | hr | 450.70 | 73.04 | p-Akt | 18.94 | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 277.93 | 72.56 | p-4EBP1 | 95.48 | | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 468.57 | 85.00 | p-Akt | 42.85 | |
| Triciribine | AU565 | 3.16 | uM | 6 | hr | 341.86 | 82.21 | p-Akt | 24.71 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 234.01 | 54.85 | p-Akt | 22.11 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 567.47 | 140.54 | p-4EBP1 | 166.16 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 659.08 | 130.41 | p-Akt | 134.35 | | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 256.25 | 58.14 | p-Akt | 13.28 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 207.40 | 54.85 | p-4EBP1 | 68.29 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 260.81 | 58.97 | p-Akt | 13.43 | |
| Gefitinib | AU565 | 3.16 | uM | 24 | hr | 347.95 | 80.80 | p-Akt | 41.35 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 207.63 | 45.48 | p-Akt | 16.31 | |
| Torkinib | AU565 | 2.37 | uM | 6 | hr | 226.24 | 41.45 | p-Akt | 12.62 | |
| Torkinib | AU565 | 2.37 | uM | 24 | hr | 421.19 | 62.31 | p-Akt | 18.44 | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 522.19 | 120.45 | p-Akt | 94.97 | | |
| Torkinib | AU565 | 133.0 | nM | 24 | hr | 395.71 | 61.17 | p-Akt | 22.66 | |
| Triciribine | AU565 | 3.16 | uM | 24 | hr | 412.86 | 71.66 | p-Akt | 32.76 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 217.68 | 50.97 | p-S6 | 71.68 | |
| Triciribine | AU565 | 3.16 | nM | 24 | hr | 537.40 | 126.81 | p-S6 | 494.49 | |