Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | AU565 | 10.0 | uM | 6 | hr | 331.62 | 71.61 | p-Akt | 21.64 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 225.05 | 59.35 | p-Akt | 21.63 | |
| Dactolisib | AU565 | 10.0 | uM | 6 | hr | 278.17 | 60.68 | p-Akt | 17.28 | |
| GSK1059615 | AU565 | 3.16 | uM | 24 | hr | 357.44 | 84.22 | p-4EBP1 | 35.21 | |
| Dactolisib | AU565 | 10.0 | uM | 24 | hr | 452.82 | 70.98 | p-Akt | 21.40 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 484.33 | 118.39 | p-Akt | 103.66 | |
| Triciribine | AU565 | 3.16 | uM | 24 | hr | 407.60 | 78.72 | p-4EBP1 | 102.21 | |
| Dactolisib | AU565 | 3.16 | uM | 24 | hr | 470.31 | 69.46 | p-4EBP1 | 9.61 | |
| Gefitinib | AU565 | 10.0 | uM | 6 | hr | 216.77 | 47.93 | p-4EBP1 | 49.07 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Torkinib | AU565 | 10.0 | uM | 24 | hr | 429.86 | 78.50 | p-4EBP1 | 15.32 | |
| GSK1059615 | AU565 | 10.0 | uM | 24 | hr | 316.18 | 83.54 | p-4EBP1 | 15.50 | |
| MK2206 | AU565 | 10.0 | uM | 6 | hr | 311.09 | 61.50 | p-4EBP1 | 70.16 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 494.41 | 109.15 | p-Akt | 109.06 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 221.28 | 52.62 | p-Akt | 23.51 | |
| Gefitinib | AU565 | 100.0 | nM | 24 | hr | 438.46 | 106.15 | p-Akt | 119.62 | |
| Torkinib | AU565 | 31.6 | nM | 6 | hr | 280.52 | 52.21 | p-Akt | 15.00 | |
| Torkinib | AU565 | 31.6 | nM | 24 | hr | 412.00 | 70.44 | p-Akt | 34.30 | |
| GSK1059615 | AU565 | 100.0 | nM | 6 | hr | 223.60 | 51.25 | p-Akt | 23.72 | |
| GSK1059615 | AU565 | 100.0 | nM | 24 | hr | 496.50 | 107.87 | p-Akt | 88.78 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 217.60 | 53.69 | p-Akt | 13.47 | |
| MK2206 | AU565 | 100.0 | nM | 24 | hr | 334.04 | 92.53 | p-Akt | 29.08 | |
| Triciribine | AU565 | 100.0 | nM | 6 | hr | 301.03 | 74.23 | p-Akt | 21.74 | |
| Triciribine | AU565 | 0 (control) | 24 | hr | 513.50 | 123.82 | p-S6 | 483.08 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 344.99 | 87.13 | p-S6 | 242.25 | |