Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 597.04 | 145.45 | p-4EBP1 | 170.31 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 346.86 | 73.14 | p-4EBP1 | 98.91 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 261.27 | 65.61 | p-4EBP1 | 75.05 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 424.14 | 125.45 | p-4EBP1 | 174.34 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 492.56 | 131.78 | p-4EBP1 | 221.65 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 192.39 | 39.79 | p-4EBP1 | 52.60 | |
| Gefitinib | HCC1954 | 1.0 | nM | 24 | hr | 286.14 | 129.34 | p-4EBP1 | 149.15 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 373.59 | 94.92 | p-4EBP1 | 112.38 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 24 | hr | 310.46 | 118.73 | p-4EBP1 | 119.18 | |
| MK2206 | HCC1954 | 1.0 | nM | 24 | hr | 287.93 | 122.38 | p-4EBP1 | 149.60 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 235.54 | 85.78 | p-4EBP1 | 104.82 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 226.23 | 50.86 | p-4EBP1 | 51.79 | | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 597.19 | 141.39 | p-4EBP1 | 180.42 | | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 520.08 | 134.68 | p-4EBP1 | 226.94 | | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 234.34 | 56.31 | p-4EBP1 | 61.94 | | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 443.02 | 135.36 | p-4EBP1 | 212.85 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 277.93 | 72.56 | p-4EBP1 | 95.48 | | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 223.15 | 50.37 | p-4EBP1 | 55.92 | | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 527.81 | 136.32 | p-4EBP1 | 202.58 | | |
| Torkinib | HCC1954 | 0 (control) | 24 | hr | 299.58 | 124.61 | p-4EBP1 | 139.95 | | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 337.11 | 90.46 | p-4EBP1 | 107.99 | | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 142.39 | 29.98 | p-4EBP1 | 49.89 | | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 452.64 | 159.11 | p-4EBP1 | 351.84 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 300.92 | 152.97 | p-4EBP1 | 337.34 | |