Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | T47D | 0 (control) | 24 | hr | 158.39 | 110.94 | p-4EBP1 | 415.89 | | |
| GSK1059615 | T47D | 0 (control) | 24 | hr | 164.30 | 107.15 | p-4EBP1 | 431.40 | | |
| MK2206 | T47D | 0 (control) | 6 | hr | 108.26 | 31.89 | p-4EBP1 | 66.68 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.03 | 45.53 | p-4EBP1 | 121.29 | | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 136.31 | 100.86 | p-4EBP1 | 345.05 | | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 597.04 | 145.45 | p-4EBP1 | 170.31 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 346.86 | 73.14 | p-4EBP1 | 98.91 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 424.14 | 125.45 | p-4EBP1 | 174.34 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 291.94 | 77.13 | p-4EBP1 | 98.69 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 492.56 | 131.78 | p-4EBP1 | 221.65 | |
| Torkinib | HCC1954 | 0.1 | nM | 24 | hr | 268.67 | 120.63 | p-4EBP1 | 124.48 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 373.59 | 94.92 | p-4EBP1 | 112.38 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 194.30 | 65.17 | p-4EBP1 | 85.41 | |
| MK2206 | HCC1954 | 1.0 | nM | 24 | hr | 287.93 | 122.38 | p-4EBP1 | 149.60 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 167.72 | 46.85 | p-4EBP1 | 64.61 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 301.34 | 115.11 | p-4EBP1 | 140.89 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 300.92 | 152.97 | p-4EBP1 | 337.34 | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 452.64 | 159.11 | p-4EBP1 | 351.84 | |
| MK2206 | MCF 10A | 1.0 | nM | 6 | hr | 424.87 | 161.04 | p-4EBP1 | 383.69 | |
| MK2206 | MCF 10A | 1.0 | nM | 24 | hr | 277.61 | 143.74 | p-4EBP1 | 324.15 | |
| Triciribine | MCF 10A | 1.0 | nM | 24 | hr | 266.33 | 138.95 | p-4EBP1 | 314.28 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 412.87 | 154.76 | p-4EBP1 | 376.60 | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 438.38 | 64.12 | p-4EBP1 | 13.69 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 328.87 | 65.51 | p-4EBP1 | 9.30 | |