Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | AU565 | 7.5 | nM | 6 | hr | 237.10 | 47.79 | p-4EBP1 | 50.80 | |
| GSK1059615 | AU565 | 31.6 | nM | 24 | hr | 464.06 | 126.07 | p-4EBP1 | 178.40 | |
| MK2206 | AU565 | 31.6 | nM | 6 | hr | 255.74 | 64.02 | p-4EBP1 | 86.03 | |
| Triciribine | AU565 | 31.6 | nM | 6 | hr | 349.86 | 83.99 | p-4EBP1 | 125.84 | |
| Triciribine | AU565 | 31.6 | nM | 24 | hr | 499.46 | 124.57 | p-4EBP1 | 196.58 | |
| Gefitinib | HCC1954 | 31.6 | nM | 24 | hr | 270.46 | 128.49 | p-4EBP1 | 136.05 | |
| Torkinib | HCC1954 | 7.5 | nM | 6 | hr | 176.66 | 32.26 | p-4EBP1 | 41.13 | |
| GSK1059615 | HCC1954 | 31.6 | nM | 6 | hr | 274.65 | 82.84 | p-4EBP1 | 93.37 | |
| GSK1059615 | HCC1954 | 31.6 | nM | 24 | hr | 288.77 | 118.12 | p-4EBP1 | 126.97 | |
| Triciribine | HCC1954 | 31.6 | nM | 6 | hr | 250.28 | 91.52 | p-4EBP1 | 117.60 | |
| Triciribine | HCC1954 | 31.6 | nM | 24 | hr | 268.23 | 117.03 | p-4EBP1 | 128.35 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 259.09 | 89.65 | p-4EBP1 | 38.06 | |
| Gefitinib | MCF 10A | 31.6 | nM | 6 | hr | 404.00 | 166.61 | p-4EBP1 | 295.81 | |
| GSK1059615 | MCF 10A | 31.6 | nM | 24 | hr | 290.16 | 157.18 | p-4EBP1 | 233.47 | |
| MK2206 | MCF 10A | 31.6 | nM | 6 | hr | 375.80 | 155.36 | p-4EBP1 | 359.32 | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 379.12 | 156.82 | p-4EBP1 | 360.76 | |
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 237.54 | 158.78 | p-4EBP1 | 206.20 | |
| Gefitinib | T47D | 31.6 | nM | 6 | hr | 161.99 | 98.63 | p-4EBP1 | 479.28 | |
| Gefitinib | T47D | 31.6 | nM | 24 | hr | 141.14 | 119.21 | p-4EBP1 | 409.49 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 135.75 | 78.36 | p-4EBP1 | 306.59 | |
| GSK1059615 | T47D | 31.6 | nM | 6 | hr | 169.81 | 82.41 | p-4EBP1 | 499.15 | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 107.57 | 58.92 | p-4EBP1 | 199.41 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 160.29 | 105.55 | p-4EBP1 | 367.55 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 498.81 | 112.95 | p-4EBP1 | 174.19 | |
| Triciribine | AU565 | 100.0 | nM | 6 | hr | 214.70 | 54.97 | p-4EBP1 | 65.17 | |