Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | HCC1954 | 3.16 | nM | 24 | hr | 280.64 | 118.51 | p-4EBP1 | 139.15 | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 247.11 | 85.47 | p-4EBP1 | 110.31 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 471.12 | 120.05 | p-4EBP1 | 173.15 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 266.08 | 62.37 | p-4EBP1 | 79.48 | |
| Dactolisib | HCC1954 | 10.0 | uM | 6 | hr | 280.52 | 78.67 | p-4EBP1 | 11.05 | |
| Dactolisib | HCC1954 | 10.0 | uM | 24 | hr | 271.90 | 91.90 | p-4EBP1 | 12.03 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 325.09 | 126.50 | p-S6 | 407.13 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 409.16 | 66.25 | p-S6 | 240.60 | |
| Dactolisib | T47D | 1.0 | nM | 24 | hr | 143.56 | 106.16 | p-4EBP1 | 333.82 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 92.26 | 27.93 | p-4EBP1 | 75.18 | |
| Dactolisib | MCF 10A | 1.0 | nM | 24 | hr | 262.81 | 138.59 | p-4EBP1 | 280.35 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 422.75 | 163.30 | p-4EBP1 | 379.78 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 273.28 | 115.82 | p-4EBP1 | 137.09 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 250.11 | 85.06 | p-4EBP1 | 113.97 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 497.45 | 123.10 | p-4EBP1 | 179.16 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 260.00 | 60.48 | p-4EBP1 | 77.46 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 286.54 | 126.04 | p-4EBP1 | 6.41 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 436.42 | 67.63 | p-4EBP1 | 9.79 | |
| Dactolisib | T47D | 316.0 | nM | 24 | hr | 116.03 | 41.14 | p-Akt | 14.73 | |
| Dactolisib | T47D | 316.0 | nM | 6 | hr | 92.43 | 45.00 | p-Akt | 6.96 | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 158.04 | 108.23 | p-4EBP1 | 356.79 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 88.35 | 28.53 | p-4EBP1 | 84.26 | | |
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 265.37 | 131.31 | p-4EBP1 | 328.31 | | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 444.47 | 164.51 | p-4EBP1 | 404.34 | | |
| Dactolisib | HCC1954 | 0 (control) | 24 | hr | 304.21 | 127.11 | p-4EBP1 | 143.88 | |