Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 567.47 | 140.54 | p-4EBP1 | 166.16 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 567.06 | 138.57 | p-4EBP1 | 168.25 | | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 566.41 | 105.22 | p-S6 | 313.45 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 565.94 | 130.77 | p-S6 | 519.83 | | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 565.14 | 135.83 | p-4EBP1 | 162.84 | |
| Torkinib | MCF 10A | 10.0 | uM | 6 | hr | 563.34 | 144.82 | p-Akt | 16.90 | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 562.01 | 105.00 | p-4EBP1 | 155.98 | | |
| Torkinib | MCF 10A | 2.37 | uM | 6 | hr | 561.92 | 142.49 | p-Akt | 17.93 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 560.89 | 123.46 | p-S6 | 526.76 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 559.78 | 127.56 | p-S6 | 500.36 | |
| Triciribine | AU565 | 31.6 | nM | 24 | hr | 558.65 | 122.04 | p-S6 | 463.83 | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 557.64 | 128.55 | p-S6 | 521.30 | | |
| Torkinib | MCF 10A | 2.37 | uM | 24 | hr | 557.47 | 88.94 | p-S6 | 320.73 | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 556.41 | 101.00 | p-Akt | 80.71 | | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 556.22 | 122.40 | p-Akt | 136.15 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 553.63 | 148.24 | p-4EBP1 | 36.47 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 552.62 | 106.48 | p-Akt | 64.22 | |
| Triciribine | AU565 | 10.0 | nM | 24 | hr | 550.75 | 128.39 | p-S6 | 471.06 | |
| Gefitinib | AU565 | 10.0 | nM | 24 | hr | 550.65 | 128.14 | p-4EBP1 | 151.56 | |
| Torkinib | MCF 10A | 133.0 | nM | 6 | hr | 548.44 | 168.01 | p-S6 | 609.66 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 548.27 | 160.67 | p-4EBP1 | 34.82 | |
| Torkinib | MCF 10A | 10.0 | uM | 6 | hr | 545.59 | 138.86 | p-4EBP1 | 8.61 | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 545.09 | 120.07 | p-Akt | 140.76 | | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 545.03 | 125.22 | p-Akt | 105.22 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 543.48 | 156.73 | p-S6 | 529.47 | |