Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | AU565 | 1.78 | nM | 24 | hr | 457.35 | 107.85 | p-S6 | 384.28 | |
| GSK1059615 | AU565 | 100.0 | nM | 24 | hr | 456.57 | 100.82 | p-S6 | 392.83 | |
| Triciribine | AU565 | 1.0 | uM | 24 | hr | 456.10 | 90.01 | p-4EBP1 | 126.32 | |
| GSK1059615 | AU565 | 1.0 | uM | 24 | hr | 455.83 | 72.74 | p-S6 | 22.95 | |
| Torkinib | MCF 10A | 7.5 | nM | 24 | hr | 455.61 | 175.00 | p-S6 | 479.38 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 24 | hr | 455.51 | 172.86 | p-S6 | 451.67 | |
| Gefitinib | AU565 | 316.0 | nM | 24 | hr | 455.36 | 96.01 | p-Akt | 108.37 | |
| Dactolisib | AU565 | 10.0 | nM | 24 | hr | 455.18 | 96.67 | p-S6 | 74.99 | |
| Torkinib | MCF 10A | 0.422 | nM | 24 | hr | 454.80 | 162.47 | p-S6 | 458.78 | |
| Torkinib | MCF 10A | 7.5 | nM | 6 | hr | 454.63 | 172.76 | p-4EBP1 | 304.51 | |
| GSK1059615 | MCF 10A | 0 (control) | 6 | hr | 454.39 | 167.02 | p-4EBP1 | 405.75 | | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 454.27 | 168.14 | p-4EBP1 | 359.55 | |
| GSK1059615 | AU565 | 100.0 | nM | 24 | hr | 453.87 | 115.71 | p-4EBP1 | 166.38 | |
| Gefitinib | MCF 10A | 0 (control) | 6 | hr | 453.40 | 162.25 | p-4EBP1 | 316.67 | | |
| GSK1059615 | MCF 10A | 10.0 | nM | 6 | hr | 453.23 | 198.94 | p-S6 | 918.10 | |
| GSK1059615 | HCC1954 | 31.6 | nM | 6 | hr | 453.20 | 97.17 | p-Akt | 46.95 | |
| Dactolisib | AU565 | 10.0 | uM | 24 | hr | 452.82 | 70.98 | p-Akt | 21.40 | |
| GSK1059615 | AU565 | 31.6 | nM | 24 | hr | 452.71 | 121.60 | p-4EBP1 | 183.35 | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 452.66 | 116.02 | p-Akt | 85.08 | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 452.64 | 159.11 | p-4EBP1 | 351.84 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 452.52 | 107.67 | p-S6 | 260.74 | |
| Torkinib | MCF 10A | 1.78 | nM | 6 | hr | 452.12 | 168.36 | p-4EBP1 | 335.74 | |
| GSK1059615 | MCF 10A | 0 (control) | 24 | hr | 452.11 | 153.53 | p-S6 | 374.41 | | |
| GSK1059615 | AU565 | 0 (control) | 24 | hr | 452.11 | 110.23 | p-Akt | 88.09 | | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 451.76 | 117.41 | p-S6 | 545.16 | |