Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 365.11 | 76.85 | p-S6 | 156.15 | |
| Triciribine | T47D | 316.0 | nM | 24 | hr | 167.32 | 71.34 | p-Akt | 37.26 | |
| GSK1059615 | AU565 | 316.0 | nM | 24 | hr | 487.90 | 88.32 | p-S6 | 182.34 | |
| MK2206 | T47D | 316.0 | nM | 6 | hr | 106.87 | 44.20 | p-4EBP1 | 121.81 | |
| Triciribine | HCC1954 | 316.0 | nM | 6 | hr | 260.64 | 86.03 | p-4EBP1 | 124.51 | |
| GSK1059615 | AU565 | 316.0 | nM | 24 | hr | 474.59 | 87.91 | p-Akt | 63.03 | |
| MK2206 | HCC1954 | 316.0 | nM | 6 | hr | 189.81 | 54.51 | p-4EBP1 | 76.66 | |
| Triciribine | HCC1954 | 316.0 | nM | 24 | hr | 241.93 | 108.12 | p-4EBP1 | 118.63 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 6 | hr | 293.57 | 165.75 | p-S6 | 345.88 | |
| Gefitinib | MCF 10A | 316.0 | nM | 6 | hr | 223.68 | 160.02 | p-S6 | 420.56 | |
| GSK1059615 | AU565 | 316.0 | nM | 6 | hr | 232.75 | 48.96 | p-Akt | 23.52 | |
| Gefitinib | MCF 10A | 316.0 | nM | 24 | hr | 262.25 | 189.00 | p-S6 | 404.58 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 24 | hr | 306.73 | 152.32 | p-S6 | 257.52 | |
| MK2206 | MCF 10A | 316.0 | nM | 6 | hr | 402.65 | 157.16 | p-S6 | 488.56 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 328.87 | 65.51 | p-4EBP1 | 9.30 | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 499.67 | 91.10 | p-4EBP1 | 143.95 | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 438.38 | 64.12 | p-4EBP1 | 13.69 | |
| MK2206 | AU565 | 316.0 | nM | 24 | hr | 320.76 | 76.81 | p-Akt | 22.30 | |
| Triciribine | AU565 | 316.0 | nM | 6 | hr | 374.37 | 86.05 | p-4EBP1 | 118.53 | |
| Gefitinib | HCC1954 | 316.0 | nM | 6 | hr | 166.89 | 61.52 | p-4EBP1 | 80.63 | |
| Triciribine | HCC1954 | 316.0 | nM | 24 | hr | 247.45 | 107.48 | p-S6 | 333.56 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 333.41 | 71.95 | p-4EBP1 | 113.09 | |
| GSK1059615 | AU565 | 316.0 | nM | 24 | hr | 482.72 | 91.32 | p-4EBP1 | 156.20 | |
| MK2206 | AU565 | 316.0 | nM | 24 | hr | 342.39 | 86.36 | p-4EBP1 | 122.77 | |
| GSK1059615 | HCC1954 | 316.0 | nM | 6 | hr | 348.89 | 81.09 | p-Akt | 24.10 | |