Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Torkinib | T47D | 10.0 | uM | 6 | hr | 166.48 | 70.30 | p-4EBP1 | 22.16 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 123.65 | 43.82 | p-4EBP1 | 9.57 | |
| GSK1059615 | T47D | 10.0 | uM | 24 | hr | 241.99 | 78.61 | p-4EBP1 | 83.28 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 108.84 | 30.13 | p-4EBP1 | 73.21 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 234.22 | 57.81 | p-Akt | 20.79 | |
| Gefitinib | AU565 | 1.0 | uM | 24 | hr | 360.24 | 81.24 | p-Akt | 78.24 | |
| GSK1059615 | AU565 | 1.0 | uM | 24 | hr | 419.10 | 71.76 | p-Akt | 30.83 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 260.81 | 58.97 | p-Akt | 13.43 | |
| Triciribine | AU565 | 1.0 | uM | 24 | hr | 442.50 | 77.06 | p-Akt | 37.03 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 327.69 | 72.03 | p-Akt | 17.75 | |
| Gefitinib | HCC1954 | 1.0 | uM | 6 | hr | 135.01 | 33.51 | p-Akt | 20.62 | |
| Gefitinib | HCC1954 | 1.0 | uM | 24 | hr | 240.65 | 87.74 | p-Akt | 64.52 | |
| MK2206 | HCC1954 | 1.0 | uM | 24 | hr | 242.00 | 86.91 | p-Akt | 18.32 | |
| Triciribine | HCC1954 | 1.0 | uM | 6 | hr | 218.47 | 78.02 | p-Akt | 28.41 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 235.17 | 64.66 | p-Akt | 16.95 | |
| Dactolisib | HCC1954 | 1.0 | uM | 24 | hr | 288.44 | 57.74 | p-Akt | 18.48 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 6 | hr | 215.88 | 128.98 | p-Akt | 16.35 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 24 | hr | 270.51 | 77.62 | p-Akt | 15.17 | |
| MK2206 | MCF 10A | 1.0 | uM | 24 | hr | 305.61 | 145.88 | p-Akt | 15.53 | |
| Triciribine | MCF 10A | 1.0 | uM | 6 | hr | 373.41 | 152.18 | p-Akt | 19.21 | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 156.94 | 108.01 | p-Akt | 80.72 | |
| GSK1059615 | T47D | 1.0 | uM | 6 | hr | 158.41 | 63.89 | p-Akt | 21.30 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 107.58 | 49.92 | p-Akt | 7.19 | |
| GSK1059615 | AU565 | 1.0 | uM | 6 | hr | 237.78 | 52.90 | p-S6 | 9.57 | |
| Gefitinib | AU565 | 1.0 | uM | 24 | hr | 361.65 | 88.03 | p-S6 | 303.58 | |