Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | HCC1954 | 100.0 | nM | 6 | hr | 161.80 | 35.80 | p-S6 | 8.52 | |
| Dactolisib | HCC1954 | 100.0 | nM | 24 | hr | 277.05 | 85.11 | p-S6 | 17.62 | |
| Gefitinib | MCF 10A | 100.0 | nM | 24 | hr | 372.85 | 195.99 | p-S6 | 350.71 | |
| Torkinib | MCF 10A | 31.6 | nM | 6 | hr | 512.25 | 160.72 | p-S6 | 563.19 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 305.86 | 66.00 | p-S6 | 165.72 | | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 612.84 | 138.04 | p-S6 | 527.40 | | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 557.64 | 128.55 | p-S6 | 521.30 | | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 265.42 | 70.65 | p-S6 | 168.80 | | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 458.85 | 128.23 | p-S6 | 525.22 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 344.99 | 87.13 | p-S6 | 242.25 | | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 246.18 | 58.17 | p-S6 | 130.86 | | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 529.58 | 125.56 | p-S6 | 517.92 | | |
| Torkinib | HCC1954 | 0 (control) | 24 | hr | 308.22 | 114.54 | p-S6 | 428.36 | | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 398.51 | 94.64 | p-S6 | 384.85 | | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 248.38 | 75.69 | p-S6 | 177.47 | | |
| MK2206 | HCC1954 | 0 (control) | 24 | hr | 305.43 | 118.14 | p-S6 | 334.28 | | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 182.67 | 48.86 | p-S6 | 100.01 | | |
| Dactolisib | HCC1954 | 0 (control) | 24 | hr | 328.01 | 114.08 | p-S6 | 351.21 | | |
| Gefitinib | MCF 10A | 0 (control) | 24 | hr | 338.27 | 145.29 | p-S6 | 360.92 | | |
| Torkinib | MCF 10A | 0 (control) | 6 | hr | 487.61 | 155.47 | p-S6 | 474.69 | | |
| MK2206 | MCF 10A | 0 (control) | 24 | hr | 356.25 | 145.59 | p-S6 | 253.80 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 446.41 | 161.85 | p-S6 | 482.53 | | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 464.95 | 166.23 | p-S6 | 565.63 | | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 379.04 | 131.01 | p-S6 | 368.12 | |
| Triciribine | MCF 10A | 100.0 | nM | 24 | hr | 252.76 | 159.17 | p-S6 | 232.52 | |