Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | HCC1954 | 316.0 | nM | 6 | hr | 169.38 | 48.39 | p-Akt | 24.53 | |
| Gefitinib | HCC1954 | 316.0 | nM | 24 | hr | 250.36 | 103.57 | p-Akt | 72.98 | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 174.96 | 31.04 | p-Akt | 14.51 | |
| Torkinib | HCC1954 | 133.0 | nM | 24 | hr | 234.19 | 75.17 | p-Akt | 27.06 | |
| GSK1059615 | HCC1954 | 316.0 | nM | 6 | hr | 348.89 | 81.09 | p-Akt | 24.10 | |
| GSK1059615 | HCC1954 | 316.0 | nM | 24 | hr | 303.70 | 70.83 | p-Akt | 23.41 | |
| MK2206 | HCC1954 | 316.0 | nM | 6 | hr | 223.77 | 64.61 | p-Akt | 15.70 | |
| MK2206 | HCC1954 | 316.0 | nM | 24 | hr | 244.70 | 93.44 | p-Akt | 20.33 | |
| Triciribine | HCC1954 | 316.0 | nM | 6 | hr | 241.79 | 82.96 | p-Akt | 31.94 | |
| Triciribine | HCC1954 | 316.0 | nM | 24 | hr | 241.42 | 93.99 | p-Akt | 30.59 | |
| Dactolisib | HCC1954 | 316.0 | nM | 6 | hr | 172.52 | 45.49 | p-Akt | 15.11 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 255.42 | 63.44 | p-Akt | 17.92 | |
| Gefitinib | MCF 10A | 316.0 | nM | 6 | hr | 215.43 | 158.10 | p-Akt | 29.68 | |
| Gefitinib | MCF 10A | 316.0 | nM | 24 | hr | 199.50 | 113.21 | p-Akt | 24.25 | |
| Torkinib | MCF 10A | 133.0 | nM | 6 | hr | 466.79 | 144.13 | p-Akt | 22.20 | |
| Torkinib | MCF 10A | 133.0 | nM | 24 | hr | 395.84 | 135.47 | p-Akt | 17.01 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 6 | hr | 259.69 | 149.07 | p-Akt | 21.21 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 24 | hr | 297.05 | 126.32 | p-Akt | 20.34 | |
| MK2206 | MCF 10A | 316.0 | nM | 6 | hr | 361.83 | 149.71 | p-Akt | 20.90 | |
| MK2206 | MCF 10A | 316.0 | nM | 24 | hr | 302.86 | 152.87 | p-Akt | 19.19 | |
| Triciribine | MCF 10A | 316.0 | nM | 6 | hr | 364.57 | 150.90 | p-Akt | 21.02 | |
| Triciribine | MCF 10A | 316.0 | nM | 24 | hr | 232.57 | 125.08 | p-Akt | 18.69 | |
| Dactolisib | MCF 10A | 316.0 | nM | 6 | hr | 305.65 | 142.81 | p-Akt | 19.28 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 331.36 | 49.75 | p-Akt | 15.70 | |
| Gefitinib | T47D | 316.0 | nM | 6 | hr | 157.11 | 101.80 | p-Akt | 102.46 | |