MDAMB330 - Cell Line (ID:51139)
| HMS LINCS ID: | 51139 |
| Name: | MDAMB330 |
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| Parent Cell Line: | |
| Reference Source: | ATCC HTB-127 |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | epithelial |
| Details of Cell Type: | |
| Donor Sex: | female |
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| Disease: | |
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| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | 1330941 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From the vendor: Cells are cultured in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with: 30 ng/ ml human recombinant EGF, 0.016 mg/ml human recombinant insulin, 2mM glutathione, 20% fetal bovine serum. Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. A subcultivation ratio of 1:2 to 1:4 is recommended. Maintain cultures at 37˚C free of CO2. |
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| Date Publicly Available: | 2020-03-02 |
| Most Recent Update: | 2020-03-02 |
Batch Information for HMSL51139-1:
| HMS LINCS Batch ID: | 51139-1 |
| Provider: | ATCC |
| Provider Catalog ID: | HTB-127 |
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| Date Received: | |
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| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2020-03-02 |
| Most Recent Update: | 2020-03-02 |