| HMS LINCS ID:
|
51131
|
| Name:
|
184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry
|
| Alternative Names:
|
|
| LINCS ID:
|
|
| Alternative ID:
|
CLO_0001137
|
| Parent Cell Line:
|
HMSL51088-3: 184A1
|
| Reference Source:
|
Somponnat Sampattavanich (Mahidol University)
|
| Organism:
|
Homo sapiens
|
| Organ:
|
breast
|
| Tissue:
|
|
| Cell Type:
|
|
| Details of Cell Type:
|
|
| Donor Sex:
|
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
|
| Details of Disease:
|
|
| Production Details:
|
Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A
|
| Genetic Modification(s):
|
pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2017-05-02
|
| Most Recent Update:
|
2017-05-02
|