| HMS LINCS ID:
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51116
|
| Name:
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EFM-192C
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0002891
|
| Parent Cell Line:
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| Reference Source:
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Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311
|
| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
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| Cell Type:
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epithelioid
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| Details of Cell Type:
|
|
| Donor Sex:
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female
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| Donor Age:
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46
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| Donor Ethnicity:
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Caucasian
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| Donor Health Status:
|
|
| Disease:
|
DOID:3458, breast adenocarcinoma
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| Details of Disease:
|
breast adenocarcinoma
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| Production Details:
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From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma
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| Genetic Modification(s):
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none
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| Known Mutations:
|
ERBB2 amplification
|
| Citation Information for Mutations:
|
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311
|
| Verification Reference Profile:
|
|
| Growth Properties:
|
adherent; From DSMZ: epitheloid adherent cells growing as monolayers
|
| Recommended Culture Conditions:
|
From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Optimal split ratio is 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 5-8 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask. Incubation: at 37°C with 5% CO2. Doubling time: ca. 4 days.
|
| Relevant Citations:
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| Usage Note:
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| Comments:
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| Date Publicly Available:
|
2016-08-24
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| Most Recent Update:
|
2017-05-02
|