ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488 - Antibody (ID:80012)

HMS LINCS ID: 80012
Name: ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488
Alternative Names: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4344
Clone Name: D13.14.4E
Resource Identification Initiative ID: AB_2139960
LINCS ID:
Type: natural
Nominal Protein Target(s): ERK-1 (pT202; pY204)
Nominal Protein Target HMS LINCS ID(s): 201303
Nominal Non-protein Target:
Organism of Nominal Target: Homo sapiens
Other Target Information: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. Species predicted to react based on 100% sequence homology: Chicken, C. elegans. (source: Cell Signaling Technology)
Other Human Protein Target HMS LINCS ID(s): 201304; 201337; 201338
Immunogen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase. (source: Cell Signaling Technology)
Immunogen Sequence:
Fc Domain Source Species: rabbit
Clonality: monoclonal
Fc Domain Isotype: IgG
Source Organism for Production: rabbit
Production Details:
Conjugate or Label: Alexa Fluor 488
Labeling Details: The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6. (source: Cell Signaling Technology)
Relevant Citations:
Date Publicly Available: 2016-02-18
Most Recent Update: 2017-05-12

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20267
Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors
Microscopy/Imaging
20303
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 1 of 6: Cytosolic fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20304
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 2 of 6: Nuclear fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20305
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20306
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 4 of 6: Nuclear fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20307
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 5 of 6: Cytosolic fluorescent intensity measurements at 72 hr.
Microscopy/Imaging
20308
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 6 of 6: Nuclear fluorescent intensity measurements at 72 hr.
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
80012-1 Cell Signaling Technology 4344S 12