Cor.4U - Differentiated Cell (ID:75001)
| HMS LINCS ID: | 75001 |
| Name: | Cor.4U |
| Alternative Names: | Cor.4U human induced Pluripotent Stem Cell-derived cardiomyocytes (hiPSC-CMs) |
| LINCS ID: | |
| Alternative ID: | |
| Precursor iPSC Cell: | HMSL70001-1: Axiogenesis iPSC |
| Details of Cell Type: | cardiomyocyte |
| Cell Type Detail: | mix of sub-types: atrial, ventricular, nodal |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Molecular Features: | |
| Recommended Culture Conditions: |
Complete culture medium recipe: 450mL IMDM (PAN,P04-20150) 500μL Insulin (Insulin solution human, I9278, Sigma) 50mL Fetal calf serum (heat inactivated) Thioglycerol (400 μM) BMCC serum free culture medium recipe: 500ml IMDM (PAN,P04-20150) 500μl Insulin (Insulin solution human, I9278, Sigma) 5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco) 500μl Trace Elements A ( 25-021-CI, Cellgro) 500μl Trace Elements B ( 99-175-CI, Cellgro) 500μl Trace Elements C (99-176-CI, Cellgro) 250μl Vitamin C ( 100mg/ml Stock) Thioglycerol (400 μM) |
| Relevant Citations: | |
| Related Projects: | |
| Cell Markers: | express cardiac-specific proteins, e.g. cardiac alpha-actinin and the gap junction protein connexin-43 |
| Genetic Modification(s): | |
| Differentiation Protocol: | proprietary protocol |
| Comments: | |
| Date Publicly Available: | 2017-09-29 |
| Most Recent Update: | 2017-09-29 |
Batch Information for HMSL75001-2:
| HMS LINCS Batch ID: | 75001-2 |
| Provider: | Ncardia, previously Axiogenesis |
| Provider Catalog ID: | Ax-B-HC02-4M |
| Provider Batch ID: | CB212CL_V_4M |
| Source Information: | |
| Date Received: | |
| HMS QC Outcome: | |
| Culture Conditions: |
Complete culture medium recipe: 450mL IMDM (PAN,P04-20150) 500μL Insulin (Insulin solution human, I9278, Sigma) 50mL Fetal calf serum (heat inactivated) Thioglycerol (400 μM) BMCC serum free culture medium recipe: 500ml IMDM (PAN,P04-20150) 500μl Insulin (Insulin solution human, I9278, Sigma) 5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco) 500μl Trace Elements A ( 25-021-CI, Cellgro) 500μl Trace Elements B ( 99-175-CI, Cellgro) 500μl Trace Elements C (99-176-CI, Cellgro) 250μl Vitamin C ( 100mg/ml Stock) Thioglycerol (400 μM) 1% FBS |
| Passage Number: | |
| Days Post Differentiation: | |
| Transient Modification(s): | none |
| Comments: | |
| Date Publicly Available: | 2017-09-29 |
| Most Recent Update: | 2018-03-20 |