Cor.4U - Differentiated Cell (ID:75001)
| HMS LINCS ID: | 75001 |
| Name: | Cor.4U |
| Alternative Names: | Cor.4U human induced Pluripotent Stem Cell-derived cardiomyocytes (hiPSC-CMs) |
| LINCS ID: | |
| Alternative ID: | |
| Precursor iPSC Cell: | HMSL70001-1: Axiogenesis iPSC |
| Details of Cell Type: | cardiomyocyte |
| Cell Type Detail: | mix of sub-types: atrial, ventricular, nodal |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Molecular Features: | |
| Recommended Culture Conditions: |
Complete culture medium recipe: 450mL IMDM (PAN,P04-20150) 500μL Insulin (Insulin solution human, I9278, Sigma) 50mL Fetal calf serum (heat inactivated) Thioglycerol (400 μM) BMCC serum free culture medium recipe: 500ml IMDM (PAN,P04-20150) 500μl Insulin (Insulin solution human, I9278, Sigma) 5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco) 500μl Trace Elements A ( 25-021-CI, Cellgro) 500μl Trace Elements B ( 99-175-CI, Cellgro) 500μl Trace Elements C (99-176-CI, Cellgro) 250μl Vitamin C ( 100mg/ml Stock) Thioglycerol (400 μM) |
| Relevant Citations: | |
| Related Projects: | |
| Cell Markers: | express cardiac-specific proteins, e.g. cardiac alpha-actinin and the gap junction protein connexin-43 |
| Genetic Modification(s): | |
| Differentiation Protocol: | proprietary protocol |
| Comments: | |
| Date Publicly Available: | 2017-09-29 |
| Most Recent Update: | 2017-09-29 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20324 | Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - pilot dataset | RNA-Seq |
| 20325 | Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - pilot dataset (qPCR validation) | qPCR |
| 20349 | Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - Proteomics | Proteomics |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 75001-1 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | CB188CL_V_4M |
| 75001-2 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | CB212CL_V_4M |
| 75001-3 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | CB254CL_V_4M |
| 75001-4 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | CB302CL_V_4M |
| 75001-5 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | F2015-KW19CL1 |
| 75001-6 | Ncardia, previously Axiogenesis | Ax-B-HC02-4M | F2015-KW20CL2 |