RNAseq:
RNAseq_Assay_Name: RNAseq
RNAseq_Assay_Date:
RNAseq_Instrument: NextSeq
RNAseq_Software_Version:
RNAseq_Single_Paired: Single end
RNAseq_Read_Length_1: 75
RNAseq_Read_Length_2: 75
RNAseq_Strand_Specificity: Stranded
RNAseq_Library_starting_quantity: 500 ng
RNAseq_RNA_Integrity_Number: 9
RNAseq_Lysis_method: RNeasy kit
RNAseq_Extraction_method: RNeasy kit
RNAseq_Fragmentation_method: TruSeq stranded mRNA kit
RNAseq_Size_selection_method: SPRI beads
RNAseq_Size_Range: 200-500
RNAseq_Spike-in_Controls: ERCC mix 2
RNAseq_Spike-in_Source: Ambion
RNAseq_Spike-in_Concentration: 5 ul of 1:500 dilution
RNAseq_Mean_Sequencing_Depth: 6.6 x 10^6
RNAseq_Sequencing_Cycles: 75 single end
RNAseq_Reference_Genome: GRCh37
RNAseq_Trim_metric: <30 (Phred)
RNAseq_Lib_prep_kit: TruSeq stranded mRNA kit
RNAseq_Biosample_Type: cell lines
RNAseq_Biosample_summary: human breast cell lines
RNAseq_Associated_SOPs: TruSeq stranded mRNA kit
RNAseq_Replication_Type: single samples
RNAseq_Replication_Count: 1
RNAseq_Library_Prep_Date: 8/16/16; 1/8/17
RNAseq_Library_Date_Extracted: 7/28/16; 12/20/16
RNAseq_Spike-in_Time: Spike-in added to extracted RNA before library prep
RNAseq_Library_Indexing_Basepairing: 8
RNAseq_Barcode: n/a
RNAseq_cDNA_Preparation_Method: Random
RNAseq_PCR_Cycle_Number: 10
RNAseq_Sequencing_Facility: Bauer core
RNAseq_Sequencing_Chemistry_Version: v2
RNAseq_Raw_File_Type: fastq
RNAseq_Raw_File_Replicate_Number:
RNAseq_Plate_Format: 96 36
RNAseq_Temperature:
RNAseq_Complexity_Perturbagen: Multiple
RNAseq_Complexity_Concentration: Multiple
RNAseq_Complexity_Cell_Type: Multiple
RNAseq_Complexity_Cell_Density: Multiple
RNAseq_Complexity_Time_Point: Multiple
RNAseq_Complexity_Perturbagen_Number: No
RNAseq_Concentration: n/a
RNAseq_Time_Point: n/a
RNAseq Assay Metadata:
RNAseq Assay Metadata: