Fixation Time Point:
Fixation Time Point: 72 hours
Imaging Control Sample:
Imaging Control Sample: FALSE
Imaging:
Settings_Group:
Imaging_Microscope_Type: 3D
Imaging_Detector_Type: sCMOS
Imaging_Detector_Bit_Depth: 16
Imaging_Detector_Binning: 2x2
Imaging_Light_Source: Xenon
Imaging_Lens_Immersion_Type: air
Imaging_Lens_Numerical_Aperture: 0.75
Imaging_Lens_Optical_Correction:
Imaging_Magnification: 10
Imaging_Pixelsize: 1.32 um
Imaging_Z_Dimension: 1
Imaging_Z_Stepsize: n/a
Design_Group:
Imaging_Plate_Format: 384 Well
Imaging_Replication_Type: isogenic
Imaging_Replication_Count: 4
Imaging_Type: fixed
Imaging_Fixative: 4% formaldehyde
Imaging_Permeabilization_Agent: n/a
Imaging_Blocking_Agent: n/a
Incubation_Group:
Imaging_Incubator: FALSE
Imaging_Incubator_Temp: n/a
Imaging_Incubator_CO2: n/a
Imaging_Incubator_O2: n/a
Imaging_Incubator_Humidity: n/a
Time_Group:
Imaging_Live: FALSE
Imaging_Total_Time: n/a
Imaging_Time_Interval: n/a
Imaging_Total_Number: 1
Imaging_Acquisition_Group_Rounds: 2
Acquisition_Groups:
Imaging_Acquisition_Group: 1
Imaging_Channel_Count: 2
Imaging_Channel_Method: sequential
Imaging_Channels:
Imaging_Channel: 1
Imaging_Method: Fluorescence
Imaging_Excitation_Wavelength: Xenon
Imaging_Excitation_Power: n/a
Imaging_Excitation_Filter: 360 400
Imaging_Dichroic_Filter: n/a
Imaging_Emission_Filter: 410 480
Imaging_Exposure_Time: variable
Imaging_Detector_Gain: n/a
Imaging_Marker_Intrinsic: FALSE
Imaging_Marker_Antibodies: FALSE
Imaging_Marker_Compounds: TRUE
Imaging_Marker_Sample_Specification: Hoechst
Imaging_Channel: 2
Imaging_Method: Fluorescence
Imaging_Excitation_Wavelength: Xenon
Imaging_Excitation_Power: n/a
Imaging_Excitation_Filter: 620 640
Imaging_Dichroic_Filter: n/a
Imaging_Emission_Filter: 650 700
Imaging_Exposure_Time: variable
Imaging_Detector_Gain: n/a
Imaging_Marker_Intrinsic: FALSE
Imaging_Marker_Antibodies: FALSE
Imaging_Marker_Compounds: TRUE
Imaging_Marker_Sample_Specification: LDR