Breast Cancer Profiling Project, Gene Expression 1: Baseline mRNA sequencing on 35 breast cell lines - Dataset (ID:20348)

    
  RNAseq: 
    RNAseq_Assay_Name:: RNAseq 
    RNAseq_Assay_Date:: 4/19/2016; 4/20/2016 
    RNAseq_Instrument:: NextSeq 
    RNAseq_Software_Version::  
    RNAseq_Single_Paired: Paired End 
    RNAseq_Read_Length_1: 75 
    RNAseq_Read_Length_2: 75 
    RNAseq_Strand_Specificity: Stranded 
    RNAseq_Library_starting_quantity: 500ng 
    RNAseq_RNA_Integrity_Number: 9 
    RNAseq_Lysis_method: RNeasy kit 
    RNAseq_Extraction_method: RNeasy kit 
    RNAseq_Fragmentation_method: TruSeq stranded mRNA kit 
    RNAseq_Size_selection_method: SPRI beads 
    RNAseq_Size_Range: 200-500 
    RNAseq_Spike-in_Controls: ERCC mix 2 
    RNAseq_Spike-in_Source: Ambion 
    RNAseq_Spike-in_Concentration: 1 ul of 1:100 dilution; 6.67ul of 1:500 dilution 
    RNAseq_Mean_Sequencing_Depth: 27 million reads/sample; 26.5 million reads/sample 
    RNAseq_Sequencing_Cycles: 75 paired end 
    RNAseq_Reference_Genome: GRCh37 
    RNAseq_Trim_metric: <30 (Phred) 
    RNAseq_Lib_prep_kit: TruSeq stranded mRNA kit 
    RNAseq_Biosample_Type: cell lines 
    RNAseq_Biosample_summary: human breast cell lines 
    RNAseq_Associated_SOPs: TruSeq stranded mRNA kit 
    RNAseq_Replication_Type: single samples 
    RNAseq_Replication_Count: 1 
    RNAseq_Library_Prep_Date: 2/18/2016; 3/29/2016 
    RNAseq_Library_Date_Extracted: 2/12/2016; 3/11/2016 
    RNAseq_Spike-in_Time: Spike-in added to extracted RNA before library prep 
    RNAseq_Library_Indexing_Basepairing: 6 
    RNAseq_Barcode: n/a 
    RNAseq_cDNA_Preparation_Method: Random 
    RNAseq_PCR_Cycle_Number: 10 
    RNAseq_Sequencing_Facility: Bauer core 
    RNAseq_Sequencing_Chemistry_Version: v2 
    RNAseq_Raw_File_Type: fastq 
    RNAseq_Raw_File_Replicate_Number:  
    RNAseq_Plate_Format: 19; 20 
    RNAseq_Temperature:  
    RNAseq_Complexity_Perturbagen: n/a 
    RNAseq_Complexity_Concentration: n/a 
    RNAseq_Complexity_Cell_Type: Multiple 
    RNAseq_Complexity_Cell_Density: Multiple 
    RNAseq_Complexity_Time_Point: n/a 
    RNAseq_Complexity_Perturbagen_Number: n/a 
    RNAseq_Concentration: n/a 
    RNAseq_Time_Point: n/a