Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 827 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.26 NGFR rabbit IgG(H+L) / donkey NGFR 870.16
WM115-B1-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.89 NGFR rabbit IgG(H+L) / donkey NGFR 1192.22
WM115-B2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1405 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.46 NGFR rabbit IgG(H+L) / donkey NGFR 1114.77
WM115-B2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1275 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.98 NGFR rabbit IgG(H+L) / donkey NGFR 1061.29
WM115-B2-rep1
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.31 NGFR rabbit IgG(H+L) / donkey NGFR 1049.31
WM115-B2-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1272 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.57 NGFR rabbit IgG(H+L) / donkey NGFR 1020.64
WM115-B2-rep1
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1359 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.90 NGFR rabbit IgG(H+L) / donkey NGFR 1036.42
WM115-B2-rep1
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.01 NGFR rabbit IgG(H+L) / donkey NGFR 1177.58
WM115-B2-rep1
A6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.53 NGFR rabbit IgG(H+L) / donkey NGFR 1167.78
WM115-B2-rep1
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1192 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.62 NGFR rabbit IgG(H+L) / donkey NGFR 1065.34
WM115-B2-rep1
A8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1442 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.89 NGFR rabbit IgG(H+L) / donkey NGFR 1114.51
WM115-B2-rep1
A9
WM-115 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 1 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.12 NGFR rabbit IgG(H+L) / donkey NGFR 1119.43
WM115-B2-rep1
B2
WM-115 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.78 NGFR rabbit IgG(H+L) / donkey NGFR 1106.46
WM115-B2-rep1
B3
WM-115 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 1 1423 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.93 NGFR rabbit IgG(H+L) / donkey NGFR 1068.64
WM115-B2-rep1
B4
WM-115 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 1 1716 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.85 NGFR rabbit IgG(H+L) / donkey NGFR 1141.58
WM115-B2-rep1
B5
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.17 NGFR rabbit IgG(H+L) / donkey NGFR 1162.33
WM115-B2-rep1
B6
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.99 NGFR rabbit IgG(H+L) / donkey NGFR 1089.78
WM115-B2-rep1
B7
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.33 NGFR rabbit IgG(H+L) / donkey NGFR 1076.76
WM115-B2-rep1
B8
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 1 1557 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.83 NGFR rabbit IgG(H+L) / donkey NGFR 1159.27
WM115-B2-rep1
C2
WM-115 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 1 1484 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.57 NGFR rabbit IgG(H+L) / donkey NGFR 1094.92
WM115-B2-rep1
C3
WM-115 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 1 1567 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.29 NGFR rabbit IgG(H+L) / donkey NGFR 524.82
WM115-B2-rep1
C4
WM-115 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 1 1472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.85 NGFR rabbit IgG(H+L) / donkey NGFR 1074.85
WM115-B2-rep1
C5
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 1 1729 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.85 NGFR rabbit IgG(H+L) / donkey NGFR 1178.59
WM115-B2-rep1
C6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 1 1197 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.08 NGFR rabbit IgG(H+L) / donkey NGFR 701.57
WM115-B2-rep1
C7
WM-115 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 1 1541 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.07 NGFR rabbit IgG(H+L) / donkey NGFR 1112.68
WM115-B2-rep1
C8