Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 691 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.59 NGFR rabbit IgG(H+L) / donkey NGFR 497.01
WM115-A2-rep1
F7
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.79 NGFR rabbit IgG(H+L) / donkey NGFR 1051.81
WM115-A2-rep1
F8
WM-115 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 1 1089 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.86 NGFR rabbit IgG(H+L) / donkey NGFR 782.30
WM115-A2-rep1
G2
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.62 NGFR rabbit IgG(H+L) / donkey NGFR 928.34
WM115-A2-rep1
G3
WM-115 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 1 993 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.63 NGFR rabbit IgG(H+L) / donkey NGFR 855.65
WM115-A2-rep1
G4
WM-115 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.32 NGFR rabbit IgG(H+L) / donkey NGFR 760.80
WM115-A2-rep1
G5
WM-115 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 1 1043 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.10 NGFR rabbit IgG(H+L) / donkey NGFR 964.47
WM115-A2-rep1
G6
WM-115 Vemurafenib Ischemin 1.0000 2.6667
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.01 NGFR rabbit IgG(H+L) / donkey NGFR 989.25
WM115-A2-rep1
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.71 NGFR rabbit IgG(H+L) / donkey NGFR 912.06
WM115-A2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1012 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.37 NGFR rabbit IgG(H+L) / donkey NGFR 833.88
WM115-A2-rep1
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 870 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.43 NGFR rabbit IgG(H+L) / donkey NGFR 881.97
WM115-A2-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1017 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.09 NGFR rabbit IgG(H+L) / donkey NGFR 852.25
WM115-A2-rep1
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 939 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.77 NGFR rabbit IgG(H+L) / donkey NGFR 884.54
WM115-A2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 898 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.58 NGFR rabbit IgG(H+L) / donkey NGFR 797.92
WM115-A2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.83 NGFR rabbit IgG(H+L) / donkey NGFR 922.23
WM115-A2-rep1
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 909 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.83 NGFR rabbit IgG(H+L) / donkey NGFR 973.88
WM115-A2-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1040 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.93 NGFR rabbit IgG(H+L) / donkey NGFR 791.91
WM115-A2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1026 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.53 NGFR rabbit IgG(H+L) / donkey NGFR 936.58
WM115-A2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1372 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.88 NGFR rabbit IgG(H+L) / donkey NGFR 973.52
WM115-A2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.03 NGFR rabbit IgG(H+L) / donkey NGFR 965.78
WM115-A2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1422 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.16 NGFR rabbit IgG(H+L) / donkey NGFR 882.89
WM115-A2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 923.84
WM115-A2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.37 NGFR rabbit IgG(H+L) / donkey NGFR 947.23
WM115-A2-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1502 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.53 NGFR rabbit IgG(H+L) / donkey NGFR 933.73
WM115-A2-rep2
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.88 NGFR rabbit IgG(H+L) / donkey NGFR 937.20
WM115-A2-rep2
A6