Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 1 2795 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.85 NGFR rabbit IgG(H+L) / donkey NGFR 129.75
Colo858-A2-rep1
E5
COLO 858 Vemurafenib Daminozide 0.3160 2.6667
uM
 48
hr
 1 3426 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.44 NGFR rabbit IgG(H+L) / donkey NGFR 145.60
Colo858-A2-rep1
E6
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 1 2153 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.18 NGFR rabbit IgG(H+L) / donkey NGFR 12.87
Colo858-A2-rep1
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 1 3105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.79 NGFR rabbit IgG(H+L) / donkey NGFR 132.41
Colo858-A2-rep1
E8
COLO 858 Vemurafenib Decitabine 0.3160 2.6667
uM
 48
hr
 1 1469 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.32 NGFR rabbit IgG(H+L) / donkey NGFR 124.92
Colo858-A2-rep1
F2
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 1 981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.96 NGFR rabbit IgG(H+L) / donkey NGFR 147.82
Colo858-A2-rep1
F3
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 1 169 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.43 NGFR rabbit IgG(H+L) / donkey NGFR 28.52
Colo858-A2-rep1
F4
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 1 1783 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.42 NGFR rabbit IgG(H+L) / donkey NGFR 68.18
Colo858-A2-rep1
F6
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 1 1486 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.87 NGFR rabbit IgG(H+L) / donkey NGFR 7.82
Colo858-A2-rep1
F7
COLO 858 Vemurafenib CX-5461 0.3160 2.6667
uM
 48
hr
 1 1891 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.48 NGFR rabbit IgG(H+L) / donkey NGFR 203.21
Colo858-A2-rep1
F8
COLO 858 Vemurafenib RG-108 0.3160 2.6667
uM
 48
hr
 1 1471 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.76 NGFR rabbit IgG(H+L) / donkey NGFR 126.71
Colo858-A2-rep1
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.28 NGFR rabbit IgG(H+L) / donkey NGFR 120.19
Colo858-A2-rep1
G3
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.04 NGFR rabbit IgG(H+L) / donkey NGFR 114.40
Colo858-A2-rep1
G4
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 1 581 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.25 NGFR rabbit IgG(H+L) / donkey NGFR 98.55
Colo858-A2-rep1
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 1 1165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.43 NGFR rabbit IgG(H+L) / donkey NGFR 172.86
Colo858-A2-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 1 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.48 NGFR rabbit IgG(H+L) / donkey NGFR 114.56
Colo858-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 854 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.42 NGFR rabbit IgG(H+L) / donkey NGFR 127.24
Colo858-A2-rep1
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 801 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.52 NGFR rabbit IgG(H+L) / donkey NGFR 119.13
Colo858-A2-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.09 NGFR rabbit IgG(H+L) / donkey NGFR 110.85
Colo858-A2-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.98 NGFR rabbit IgG(H+L) / donkey NGFR 130.86
Colo858-A2-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 683 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.61 NGFR rabbit IgG(H+L) / donkey NGFR 110.87
Colo858-A2-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 673 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.26 NGFR rabbit IgG(H+L) / donkey NGFR 130.08
Colo858-A2-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 978 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.02 NGFR rabbit IgG(H+L) / donkey NGFR 117.92
Colo858-A2-rep1
H7