Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 3 6944 998 0.8976 0.8479 0.1437 0.1394
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 1 5723 812 0.7414 0.7333 0.1419 0.1335
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 2 5421 694 0.7136 0.7333 0.1280 0.1335
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 3 5677 742 0.7450 0.7333 0.1307 0.1335
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 3.1600
uM
 72
hr
 1 4932 1088 0.5803 0.5134 0.2206 0.2019
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 3.1600
uM
 72
hr
 2 3952 731 0.4863 0.5134 0.1850 0.2019
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 3.1600
uM
 72
hr
 3 3922 785 0.4736 0.5134 0.2002 0.2019
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.0000
uM
 72
hr
 1 1494 774 0.1087 0.0963 0.5181 0.5591
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.0000
uM
 72
hr
 2 1450 804 0.0975 0.0963 0.5545 0.5591
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.0000
uM
 72
hr
 3 1384 837 0.0826 0.0963 0.6048 0.5591
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.3160
uM
 72
hr
 1 1298 953 0.0521 0.0581 0.7342 0.7226
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.3160
uM
 72
hr
 2 1436 1042 0.0595 0.0581 0.7256 0.7226
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 0.3160
uM
 72
hr
 3 1421 1006 0.0627 0.0581 0.7080 0.7226
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 1.0000
uM
 72
hr
 1 1226 930 0.0447 0.0438 0.7586 0.7492
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 1.0000
uM
 72
hr
 2 1143 848 0.0445 0.0438 0.7419 0.7492
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 1.0000
uM
 72
hr
 3 1107 827 0.0423 0.0438 0.7471 0.7492
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 3.1600
uM
 72
hr
 1 1368 999 0.0557 0.0335 0.7303 0.8251
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 3.1600
uM
 72
hr
 2 1251 1079 0.0260 0.0335 0.8625 0.8251
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.1000 0.0100 3.1600
uM
 72
hr
 3 1064 939 0.0189 0.0335 0.8825 0.8251
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.0000
uM
 72
hr
 1 1282 812 0.0710 0.0732 0.6334 0.6424
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.0000
uM
 72
hr
 2 1340 875 0.0702 0.0732 0.6530 0.6424
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.0000
uM
 72
hr
 3 1448 928 0.0785 0.0732 0.6409 0.6424
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.3160
uM
 72
hr
 1 1283 974 0.0466 0.0470 0.7592 0.7476
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.3160
uM
 72
hr
 2 1234 945 0.0436 0.0470 0.7658 0.7476
COLO 858 Vemurafenib Trametinib JNK-IN-8 0.3160 0.0316 0.3160
uM
 72
hr
 3 1191 855 0.0507 0.0470 0.7179 0.7476