Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 7471 1087 1.0589 1.0000 0.1455 0.1402
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 7489 1113 1.0576 1.0000 0.1486 0.1402
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 6098 771 0.8836 1.0000 0.1264 0.1402
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 1 1436 410 0.1702 0.2268 0.2855 0.2365
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 1735 338 0.2317 0.2268 0.1948 0.2365
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 2178 499 0.2785 0.2268 0.2291 0.2365
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 1 1442 278 0.1931 0.2071 0.1928 0.1768
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 1561 309 0.2077 0.2071 0.1980 0.1768
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 1546 216 0.2206 0.2071 0.1397 0.1768
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 2628 502 0.3526 0.2882 0.1910 0.1748
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 2040 356 0.2793 0.2882 0.1745 0.1748
COLO 858 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 1667 265 0.2325 0.2882 0.1590 0.1748
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 1 1367 809 0.0926 0.0996 0.5918 0.5798
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 2 1504 829 0.1120 0.0996 0.5512 0.5798
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 3 1410 841 0.0944 0.0996 0.5965 0.5798
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 1 849 828 0.0035 0.0045 0.9753 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 2 975 944 0.0051 0.0045 0.9682 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 3 1204 1175 0.0048 0.0045 0.9759 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 1 959 908 0.0085 0.0066 0.9468 0.9516
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 2 683 654 0.0048 0.0066 0.9575 0.9516
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 3 805 765 0.0066 0.0066 0.9503 0.9516
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 1 1337 1219 0.0196 0.0145 0.9117 0.9231
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 2 1225 1149 0.0126 0.0145 0.9380 0.9231
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 3 846 778 0.0113 0.0145 0.9196 0.9231
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.0000
uM
 72
hr
 1 1213 808 0.0672 0.0672 0.6661 0.6856