COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 287.6 | 3 | 0172-0428 | 33.62 | 428.69 | 325 | 72.91 | 0.768 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 251.1 | 3 | 0173-0431 | 269.61 | 431.74 | 353 | 76.91 | 0.750 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 269.6 | 3 | 0174-0431 | 528.13 | 432.17 | 372 | 77.94 | 0.770 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 1270.5 | 3 | 0175-0436 | 693.67 | 436.44 | 627 | 99.64 | 0.794 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 315.5 | 3 | 0176-0447 | 421.89 | 446.54 | 748 | 106.95 | 0.822 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 289.0 | 3 | 0177-0440 | 641.30 | 440.64 | 368 | 74.57 | 0.832 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 322.2 | 3 | 0178-0446 | 162.63 | 445.35 | 712 | 105.88 | 0.798 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 2206.0 | 3 | 0179-0443 | 218.47 | 442.01 | 246 | 66.18 | 0.706 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 1773.9 | 3 | 0180-0444 | 231.04 | 446.37 | 219 | 63.70 | 0.678 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 217.8 | 3 | 0181-0448 | 196.51 | 448.67 | 338 | 72.43 | 0.810 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 275.6 | 3 | 0182-0447 | 315.10 | 447.79 | 367 | 74.91 | 0.822 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 260.3 | 3 | 0183-0452 | 248.41 | 451.51 | 349 | 71.60 | 0.856 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 299.0 | 3 | 0184-0455 | 527.89 | 453.68 | 409 | 81.84 | 0.767 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 505.3 | 3 | 0185-0461 | 105.25 | 461.70 | 375 | 81.15 | 0.716 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 256.4 | 3 | 0186-0463 | 71.14 | 463.23 | 353 | 73.26 | 0.827 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 268.1 | 3 | 0187-0465 | 761.22 | 465.32 | 369 | 71.60 | 0.905 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 359.0 | 3 | 0188-0473 | 624.44 | 473.31 | 375 | 72.43 | 0.898 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 265.1 | 3 | 0189-0476 | 471.95 | 476.54 | 334 | 72.91 | 0.790 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 322.9 | 3 | 0190-0475 | 851.66 | 474.90 | 353 | 71.01 | 0.880 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 212.3 | 3 | 0191-0476 | 125.82 | 475.90 | 352 | 77.98 | 0.727 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 1027.6 | 3 | 0192-0482 | 929.08 | 482.82 | 380 | 78.33 | 0.778 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 268.5 | 3 | 0193-0481 | 238.90 | 481.00 | 375 | 78.57 | 0.763 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 269.2 | 3 | 0194-0484 | 565.52 | 484.47 | 368 | 73.26 | 0.862 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 359.4 | 3 | 0195-0488 | 798.10 | 488.09 | 378 | 76.43 | 0.813 |
COLO 858 | Vemurafenib | 0.1 | uM | 48 | hr | | | Cycle-2 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy3 | Rb (pS807; pS811) | | 428.1 | 3 | 0196-0490 | 720.05 | 490.02 | 371 | 72.67 | 0.883 |