Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 364.6 4
0151-0297
 340.36 297.42 224 65.01 0.666
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 687.3 4
0152-0306
 848.96 306.04 363 70.43 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3146.6 4
0153-0305
 580.13 305.85 263 61.60 0.871
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 221.5 4
0154-0306
 266.38 306.65 255 63.70 0.790
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 331.0 4
0155-0304
 426.22 304.47 293 70.28 0.745
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 289.3 4
0156-0308
 771.97 308.78 268 61.60 0.888
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 284.2 4
0157-0310
 701.07 310.10 267 60.77 0.909
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2712.7 4
0158-0314
 241.79 314.07 223 55.36 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 511.2 4
0159-0318
 864.87 318.80 329 69.84 0.848
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1683.1 4
0160-0316
 426.73 316.36 194 54.87 0.810
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 291.6 4
0161-0320
 304.77 321.14 227 56.28 0.900
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1499.8 4
0162-0327
 383.06 326.66 361 74.53 0.817
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4545.9 4
0163-0332
 509.64 331.99 402 77.50 0.841
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 5094.1 4
0164-0335
 204.03 334.75 270 60.77 0.919
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1345.7 4
0165-0344
 355.36 343.79 402 75.01 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1143.9 4
0166-0339
 57.70 339.44 229 57.11 0.882
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 273.6 4
0167-0345
 257.77 345.48 367 71.01 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 13719.6 4
0168-0348
 683.97 347.91 269 62.18 0.874
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 238.1 4
0169-0349
 923.36 349.53 318 67.01 0.890
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3508.9 4
0170-0350
 306.71 349.98 262 60.77 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 415.6 4
0171-0349
 798.73 349.42 249 64.18 0.760
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 481.0 4
0172-0354
 856.66 353.38 287 62.43 0.925
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3732.8 4
0173-0354
 27.62 354.81 251 69.84 0.647
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 287.8 4
0174-0361
 69.30 360.62 363 70.77 0.911
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 437.9 4
0175-0361
 794.64 361.10 169 49.70 0.860