Live-cell imaging of cell-to-cell variability in caspase-8 FRET reporter activity and cell fate trajectory modeling - Dataset (ID:20232)
Data Columns
Download: Excel (XLSX), Comma-separated (CSV)
- 19 Data Columns
| Name | Description | Display Name | Data Type | Unit | Precision | Readout Type | Replicate | Comments |
|---|---|---|---|---|---|---|---|---|
| cell | Cell reagent | Cell | cell | |||||
| smallMolecule | Small molecule perturbagen | Small Molecule | small_molecule | |||||
| smallMolConcentration | Small Mol Concentration | Numeric | 0 | |||||
| smallMolConcUnit | Small Mol Conc Unit | Text | ||||||
| protein | Protein perturbagen | Protein | protein | |||||
| proteinConcentration | Concentration of DR4/5 agonist | Protein Concentration | Numeric | 1 | ||||
| proteinConcUnit | Protein Conc Unit | Text | ||||||
| antibodyName | Antibody Name | Text | ||||||
| antibodyConcentration | Concentration of antibody agonist of DR4 or DR5 | Antibody Concentration | Numeric | 1 | ||||
| antibodyConcUnit | Antibody Conc Unit | Text | ||||||
| 2ndAntibodyName | 2nd Antibody Name | Text | ||||||
| 2ndAntibodyConcentration | Concentration of antibody clustering agent | 2nd Antibody Concentration | Numeric | 1 | ||||
| 2ndAntibodyConcUnit | 2nd Antibody Conc Unit | Text | ||||||
| replicate | Replicate | Numeric | 0 | |||||
| meanlog10KAU | Average of the calculated log10(k) values (in arbitrary units) across all individual cells analyzed for a given treatment | Mean log10(k) (a.u.) | Numeric | 2 | ||||
| meantauMin | Average of the calculated tau values (in min) across all individual cells analyzed for a given treatment | Mean tau (min) | Numeric | 4 | ||||
| survivingFraction | Fraction of cells scored as viable 10 hours after treatment based on morphology | Surviving Fraction | Numeric | 3 | ||||
| classificationaccuracyThetaT | Fraction of cells properly classified as viable or inviable 10 hours after treatment based on FRET reporter activation | Classification Accuracy (theta_T) | Numeric | 5 | ||||
| classificationaccuracyFateBoundary | Fraction of cells properly classified as viable or inviable 10 hours after treatment based on the landscape of parameters k and tau | Classification Accuracy (fate boundary) | Numeric | 5 |
- 19 Data Columns