Live-cell imaging of cell-to-cell variability in caspase-8 FRET reporter activity and cell fate trajectory modeling - Dataset (ID:20232)

Data Columns

  • 19 Data Columns
Name Description Display Name Data Type Unit Precision Readout Type Replicate Comments
cell
Cell reagent
Cell cell
smallMolecule
Small molecule perturbagen
Small Molecule small_molecule
smallMolConcentration Small Mol Concentration Numeric 0
smallMolConcUnit Small Mol Conc Unit Text
protein
Protein perturbagen
Protein protein
proteinConcentration
Concentration of DR4/5 agonist
Protein Concentration Numeric 1
proteinConcUnit Protein Conc Unit Text
antibodyName Antibody Name Text
antibodyConcentration
Concentration of antibody agonist of DR4 or DR5
Antibody Concentration Numeric 1
antibodyConcUnit Antibody Conc Unit Text
2ndAntibodyName 2nd Antibody Name Text
2ndAntibodyConcentration
Concentration of antibody clustering agent
2nd Antibody Concentration Numeric 1
2ndAntibodyConcUnit 2nd Antibody Conc Unit Text
replicate Replicate Numeric 0
meanlog10KAU
Average of the calculated log10(k) values (in arbitrary units) across all individual cells analyzed for a given treatment
Mean log10(k) (a.u.) Numeric 2
meantauMin
Average of the calculated tau values (in min) across all individual cells analyzed for a given treatment
Mean tau (min) Numeric 4
survivingFraction
Fraction of cells scored as viable 10 hours after treatment based on morphology
Surviving Fraction Numeric 3
classificationaccuracyThetaT
Fraction of cells properly classified as viable or inviable 10 hours after treatment based on FRET reporter activation
Classification Accuracy (theta_T) Numeric 5
classificationaccuracyFateBoundary
Fraction of cells properly classified as viable or inviable 10 hours after treatment based on the landscape of parameters k and tau
Classification Accuracy (fate boundary) Numeric 5
  • 19 Data Columns